β-catenin在子宫内膜癌组织中的研究

The study on the expression of β-catenin in endometrial carcinoma tissue

目的探讨β-catenin在子宫内膜癌组织中的表达情况及其与子宫内膜癌发展及转移的关系。方法选择子宫内膜癌手术患者的手术切除标本80例及正常子宫内膜手术切除标本20例,采用免疫组织化学S-P法检测β-catenin的表达,分析其在组织中表达的差异及其与临床病理特征的关系。结果在子宫内膜癌组织中β-catenin蛋白的阳性表达率明显高于正常子宫内膜组织(P<0.05),在不同组织病理分级中的表达结果 :G1级的阳性率为86.9%,G2级的阳性表达率为80.6%,G3级的阳性表达率为42.3%,三者之间差异有统计学意义(P<0.05);子宫肌层浸润<1/2病例的阳性率为56.5%,肌层浸润≥1/2者阳性率为87.3%,两者差异有统计学意义(P<0.05);淋巴结转移阴性的病例中的阳性率为59.3%,淋巴结转移阳性者为84.6%,两者之间差异有统计学意义(P<0.05);手术病理分期Ⅰ期的阳性率为63.6%,Ⅱ期的阳性率为77.3%;Ⅲ期的阳性率为80.0%,Ⅰ期与Ⅱ期差异有统计学意义(P<0.05),Ⅱ期与Ⅲ期差异无统计学意义(P>0.05)。结论β-catenin在子宫内膜癌组织中呈高表达,且与组织分级、子宫肌层浸润及淋巴结转移有关,表明β-catenin在子宫内膜癌的发生、发展、侵袭及转移中发挥作用。

Objective To investigate the expression of β-catenin in the tissue of endometrial cancer,and to explore the relationship between the expression of β-catenin and the development,metastasis of endometrial cancer.Methods The expression of β-catenin was detected in 80 specimens of surgical ablation from the patients with endometrial carcinoma and 20 specimens of surgical ablation from healthy subjects by immunocytochemisory （SP）,and the relationship between the expression of β-catenin and the clinical pathological characteristics was analyzed.Results The expression of β-catenin in endometrial carcinoma tissue was significantly higher than that of normal endometrial tissue （P0.05）.The expression of β-catenin was different in different pathological grades.The positive rate of β-catenin expression was 86.9% in grade Ⅰ;80.6% in grade Ⅱ;42.3% in grade Ⅲ,there were significant differences among the three grades（P0.05）.The positive rate ofβ-catenin expression was 56.5% in 1/2 tissue of endometrial cancer with myometrium infiltration,which was 87.3% in ≥1/2 tissue of endometrial cancer with myometrium infiltration,there was a significant difference between the two kinds of tissues（P0.05）.The positive rate of tissue without lymph node metastasis was 59.3%,however,which with lymph node metastasis was 84.6%,there was a significant difference between them（P0.05）.The expression of β-catenin was different in different pathological stages,63.6% in stageⅠ;77.3% in stageⅡ;80.0% in stage Ⅲ,there was a significant difference between the stage Ⅰ and Ⅱ（P0.05）,there was no significast difference between stage Ⅱ and Ⅲ（P0.05）.Conclusion The β-catenin is over expressed in the tissue of endomertrium carcinoma,which is related with pathological classification,myometrium infiltration and lymph node matastasis.β-catenin playes an important role in carcinogenesis,development,infiltration and metastasis of carcinoma of endometrium.