调衡方对S180荷瘤小鼠腹水癌细胞的作用及免疫功能的影响

Tumor inhibitory and immunoloregulation effects of Tiaoheng Prescription on S180 ascites of tumor-bearing mice

目的探讨调衡方对S180荷瘤小鼠腹水癌细胞的作用及免疫功能的影响。方法将60只昆明种雄性小鼠随机分为模型组,调衡方大、中、小剂量组(按生药50、25、12.5g/kg),贞芪扶正胶囊组(0.83g/kg),热休克组,将传代培养的S180癌细胞接种于昆明种小鼠腹腔,灌胃给药12d后处死小鼠,分别抽取腹水制片,光镜下观察细胞形态,并采用免疫组织化学法检测腹水CD4、CD8表达及血清白细胞介素-10(IL-10)的活性。结果调衡方中剂量组可见腹水S180癌细胞大量坏死和凋亡,模型组对肿瘤细胞形态无明显影响;调衡方中、大剂量组腹水中CD4、CD8表达均增多(P<0.01),而热休克组则主要是CD8表达升高(P<0.05)。调衡方在25、50g/kg剂量下,对S180荷瘤小鼠外周血IL-10活性水平有抑制作用(P<0.05)。结论调衡方对小鼠腹水S180癌细胞荷瘤小鼠的抑瘤作用机制可能与抑制小鼠外周血IL-10的活性及增强CD4、CD8的表达水平有关,调衡方亦可致S180癌腹水癌细胞坏死和凋亡,机制可能与其提高细胞免疫功能,从而增强机体对肿瘤细胞的免疫杀伤能力有关,从而阻止了肿瘤细胞的侵袭转移。

Objective To investigate the mechanisms of tumor Inhibitory and immunoloregulation of ＂Tiaoheng Prescription＂ on S180 ascites of tumor-bearing mice.Methods The mouse model of S180 ascites of tumor-bearing mice were established and then the mice were given Tiaoheng Formula by intragastric administration. Twelve days later, the mice were executed and the ascites smears were prepared. The cell feature in ascites was observed under light microscope, and the expression of CD4 and CD8 on the cellular membrane of the T lymphocyte in ascites and the level of IL-10 in the peripheral blood was detected with immunohistochemical or ELISA method. Results A large amount of damaged and apoptotic S180 ascites cells were found in Tiaoheng Prescription middle dosage group, and the changes of cell feature in control group were not obvious. The expressions of CD4 and CD8 on the membrane of T cells in ascites was increased in masculine group, Tiaoheng Prescription middle and large dosage groups, and CD8 expression was increased in heat-shock group. The production of IL-10 by immunocyte obviously decreased in Tiaoheng Prescription[25、50 g/（kg·d） by gavage for 12 days]-treated group（P0.05,P0.01）.Conclusion Tiaoheng Formula can inhibit the cancer cell of S180 ascites in mice. The mechanism may be related to that Tiaoheng Formula can enhance T lymphocyte immunological function by regulating the expression of T lymphocyte CD4 and CD8 molecules in S180 ascites, inhibit the activity level of IL-10 in the peripheral blood, and to prohibit the invasion and metastasis of tumor cells.