甘油和超声波联用对豚鼠皮肤光透明效果的影响

Experimental Studies on Influence of Glycerol in Combination with Ultrasound on Optical Clearing of Cavy in Vivo

为了探讨超声波对生物组织光透明效果的影响及其可能的机理,利用光相干断层成像技术和扫描电子显微镜(SEM)对分别在自然状态下、60%甘油30 min处理和60%甘油30 min处理联合超声波15 min 3种不同条件下处理成年豚鼠腹部皮肤后的光透明效果和豚鼠皮肤表面结构进行了比较研究。结果表明,经超声波后大大提高了生物组织的光透明。通过豚鼠皮肤表面的扫描电镜图片显示,可能的机理是超声波使角质层变得疏松,使角质细胞间隙变大,细胞易于剥落,降低了表皮角质层的屏障功能,有利于光透明剂的透皮吸收,从而提高了皮肤组织的光透明。

The effect of ultrasound [surgeon-performed（SP）] on the changes in optical properties and some possible mechanism is investigated.Adult cavy＇ skin of abdomen is chosen to comparatively studied by optical coherence tomography（OCT） and scanning electronic microscope（SEM） with optical clearing agents in combination with ultrasound.The OCT results show that ultrasound enhances the optical clearing of the cavy skin greatly.The SEM results demonstrate that ultrasound disrupts the highly ordered lipid structure of stratum corneum（SC）,makes the structure of SC loose,which causes the outermost cell of SC easy to exfoliate.Meanwhile,ultrasound decreases the barrier function of skin for the transdermal Absorption of optical clearing agents（OCA）,which enhances the effect of optical clearing of skin tissue.