拟南芥CBF2基因的抗旱性研究

Study on Desiccation Tolerance of CBF2 Gene in Arabidopsis thaliana

以拟南芥为材料,根据已报道的序列设计引物克隆了CBF2基因及rd29A基因启动子,并构建了植物表达载体pBI-rd-cbf,用以转化烟草。转基因烟草的Northern杂交检测显示,30%PEG诱导条件下CBF2基因在诱导30 min之后持续较高的表达水平,且表达水平受胁迫诱导程度的影响。抗旱生理指标测定显示,干旱条件下转基因烟草植株的脯氨酸含量迅速增高,远超于野生型;而丙二醛含量的增长幅度要比野生型植株小很多,且随着胁迫时间的延长,转基因植株体内的丙二醛含量逐步趋于稳定。试验证明,CBF2基因在提高农作物的抗旱性方面具有极大的利用价值。

According to the reported sequence, the primers were designed to clone the CBF2 gene and the promoter of rd29A by using the material of Arabidopsis thaliana,at the same time, the plant expression vector pBI-rd-cbf was constructed to transform tobac- co. 30 minutes after the induction, the Northern blot analysis of transgenic tobacco showed that the expression of CBF2 gene, which in- duced by 30% PEG maintained at high level. The expression level of CBF2 gene was influenced by the level of stress-induction. Drought physiological tests showed that proline content of the transgenic tobacco plants increased much more rapidly than that in wild- type. In contrast, the growth rate of malonaldehyde is much smaller than that in the wild-type. The content of malonaldehyde in trans- genie plants had gradually stabilized as the stress time extended. Consequently, the experimental results displayed that the CBF2 gene has a great value in improving the drought resistance of plants.