重症肌无力患者Foxp3^+CD4^+CD25^+调节性T细胞与AChRAb、Titin-Ab的相关研究

Research on the relationship between Foxp3^+ CD4^+ CD25^+ Treg and AChRAb or Titin-Ab in myasthenia gravis patients

目的探讨重症肌无力(myasthenia gravis,MG)患者Foxp3^+CD4^+CD25^+调节性T细胞(Foxp3^+CD4^+CD25^+Treg)与乙酰胆碱受体抗体(AChRAb)及连接素抗体(Titin-Ab)之间的关系,进一步揭示MG的发病机制。方法采用酶联免疫吸附试验(ELISA)检测22例MG患者以及20名健康对照者血清AChRAb和Titin-Ab水平;采用流式细胞术(FCM)检测两组外周血中CD4^+CD25^+Treg的比例及其表达Foxp3的比例。结果 MG患者外周血CD4^+CD25^+Treg比例[(2.9±0.52)%]与健康对照组[(3.12±0.51)%]比较无统计学差异(P>0.05);CD4^+CD25^+Treg细胞Foxp3表达比例为(37.24±9.57)%,低于健康对照组[(58.60±4.91)%](P<0.01)。MG组CD4^+CD25^+Treg表达Foxp3比例与AChRAb、Titin-Ab水平[分别为(0.232±0.060)和(0.170±0.035)pg/mL]均呈负相关(r=-0.449,P<0.05;r=-0.691,P<0.01)。结论 Foxp3^+CD4^+CD25^+Treg细胞数目减少导致机体免疫功能缺陷是MG发病的重要环节。

Objective To explore the relation between the percentage of Foxp3＋ CD4＋ CD25＋ T regulatory cells （Foxp3＋ CD4＋ CD25＋ Treg） in peripheral blood and the serum levels of AChRAb and Titin-Ab in myasthenia gravis （MG） patients, and further to analyze the pathogenesis of MG. Methods The levels of AChRAb and Titin-Ab in the serum of 22 patients with MG and 20 healthy controls were detected by ELISA. The percentage of CD4＋ CD25＋ Treg in the peripheral blood lymphocytes and that of Foxp3＋ in CD4＋ CD25＋ Treg were detected by FCM. Results The percentage of CD4＋ CD25＋ Treg in peripheral blood was comparable between the MG group and the control group [（2.9±0.52） % vs. （3.12±0.51） %, respectively, P〉0. 05], but the expression of Foxp3 in CD4＋ CD25＋ Treg was significantly decreased in the MG group as compared to that in the control group [ （37.24±9.57）% vs. （58.60±4.91）%, respectively, P〈0.01]. There was negative correlation between the expression of Foxp3 of CD4＋ CD25＋ Treg in peripheral blood and the serum levels of AChRAb and Titin-Ab [ （0. 232±0. 060） pg/mL, （0. 170± 0. 035） pg/mL, respectively; r= -0. 449, P〈 0.05; r=- 0. 691, P〈0.01]. Conclusions The decreased number of Foxp3＋ CD4＋ CD25＋ of Treg led to immune deficiency, and that might play an important role in the pathogenesis of MG.