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牛β-酪蛋白座位无启动子基因打靶载体的构建 被引量：2

Non-Promotor Gene Targeting Vector Construction for Bovine β-casein Site
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摘要 将带有新霉素基因(neo)和绿色荧光蛋白基因(GFP)的质粒pEGFP-C1进行了改造,去掉neo基因的启动子(Psv40),在无启动子的neo和完整的GFP基因两侧分别加入一个LoxP位点。然后将带有LoxP位点、筛选标记基因和报告基因的结构装入左右分别为牛β-casein基因1590bp和5833bp的同源臂结构中,构建成无启动子基因打靶载体pT-1590-LoxP-GFP-LoxP-5833。 Plasmid vector pEGFP-C1,including neo and GFP gene,was modified. Psv40 promoter for neo was deleted,and introduce a LoxP locus into each flank of non-promoter neo and complete GFP,constructed pEGFP-LoxP. Then we introduced 1 590 bp and 5 833 bp sequences from bovine genomes into vector pEGFP-LoxP,completed construction of non-promoter gene targeting vector,which is pT-1590-LoxP-GFP-LoxP-5833,to bovine β-casein gene locus.
作者 李瑞国 苗朝华 侯健 关宏 安晓荣 陈永福
机构地区 中国生物技术发展中心 中国农业科学院生物技术研究所 中国农业大学农业生物技术国家重点实验室
出处 《华北农学报》 CSCD 北大核心 2010年第4期17-24,共8页 Acta Agriculturae Boreali-Sinica
基金 国家"863"计划项目(2002AA206311) 国家自然科学基金项目(30871783 30700573)
关键词 牛β酪蛋白 LOXP 无启动子 打靶载体 构建 Bovine β-casein LoxP Non-promoter Targeting vector Construction
分类号 Q785 [生物学—分子生物学]
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参考文献21

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共引文献7

同被引文献16

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华北农学报
2010年 第4期

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