小麦高分子量麦谷蛋白亚基基因1By8的分子检测和应用

Molecular Marker Detection and Utilization for HMW-GS 1By8 Gene in Wheat

小麦的加工品质与小麦高低分子量麦谷蛋白的组成和含量密切相关,特别是Glu-1位点编码的高分子量麦谷蛋白亚基(HMW-GS)的组成和含量。为此,试验利用1By8亚基标记对黄淮麦区目前生产利用或曾经利用过的小麦品种及部分育种资源共150份进行了检测,其中有43份材料扩增出527bp特异片段,表明具有1By8亚基;107份材料未扩增出527bp片段,表明不具有1By8亚基;1By8亚基的出现频率为28.6%。所用材料的鉴定结果与SDS-PAGE电泳的结果基本一致,表明利用特异性PCR标记鉴定1By8亚基是可靠的。与SDS-PAGE相比,PCR标记更加快速、简便和准确。

Wheat processing quality is closely correlated with composition and quantity of glutenin,in particular with the high-molecular weight glutenin subunits（HMW-GS） encoded by the Glu-1 locus. The specific PCR marker of 1By8 was applied to detect the presence of the HMW-glutenin 1By8 gene in 150 wheat varieties and lines used in Huanghuai Wheat Region. The results showed that the PCR marker could amplify a 527 bp fragment in 43 varieties （lines） and the positive control,Tasman,comprising the 1By8 gene,while other 107 varieties（lines） and the negative control（Kukri） did not show the specific product. The probability of the 1By8 gene was 28. 6% . The results corre- sponding to those by protein SDS-PAGE indicated that this marker was reliable and effective and could identify the presence of the HMW-glutenin 1By8 gene. Compared with SDS-PAGE detection,PCR is quicker ,easier and accurate.