摘要
为应用转录因子CBF4基因对苜蓿进行抗逆性改良,试验利用聚合酶链式反应技术(PCR)从拟南芥(Arabi-dopsis thaliana)植株中克隆了CBF4基因,与GenBank中基因序列同源性可达99.41%,并将该基因连接到植物表达载体PBI121中,成功构建了适于苜蓿农杆菌遗传转化的植物表达载体,为下一步利用CBF4基因快速培育耐逆性强的苜蓿新品种奠定了基础。
In order to improve the resistance of alfalfa using CBF4,the experiment has cloned CBF4 genes by PCR technique from the plants of Arabidopsis thaliana and cloned gene sequences of CBF4 had highly homologous with GenBank,reached 99. 41% respectively. Then connected the gene into the plant expression vector PBI121,Basic above all,the experiment has been success constructed the plant expression vector that suitable for the alfalfa agrobacterium genetic transformation. It is a foundation that for next step to using the CBF4 gene fast breeds new high resistance alfalfa varieties.
出处
《华北农学报》
CSCD
北大核心
2010年第4期53-56,共4页
Acta Agriculturae Boreali-Sinica
基金
中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)项目(2006-01-01)
关键词
CBF4
拟南芥
克隆
表达载体构建
CBF4
Arabidopsis thaliana
Clone
Construction of the plant expression vector
