摘要
根据GenBank上登录的甜瓜ACC氧化酶基因1(Cm-ACO1)序列,设计特异性引物,应用RT-PCR从甜瓜品种河套蜜瓜成熟果实中克隆得到了ACO1的全长cDNA,共1035bp,并对其进行了序列分析,结果表明,所克隆的cD-NA与已报道的甜瓜品种Cantaloup charentais的ACO1cDNA序列完全一致。该基因的克隆为进一步研究ACC氧化酶基因在甜瓜中的表达特性以及功能奠定了基础。
A pair of specific primer was designed based on melon ACC oxidase gene Cm-ACO1 in Genbank. The full-length cDNA of ACO1 was cloned by RT-PCR from ripening fruit of melon （Cucumis melo L. cv. Hetao） . The length of cDNA was 1 035 bp. Sequence analysis indicated that the cDNA sequence was consistent with that of reported melon ACO1 cDNA. This work may contribute to study the expression pattern and function of ACO1 in melon.
出处
《华北农学报》
CSCD
北大核心
2010年第4期61-63,共3页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金资助项目(30660111)
国家基础科学人才培养基金资助项目(J0730648)
