莱芜猪和杜洛克猪肌肉H-FABP基因表达量与肌内脂肪和脂肪酸含量关联分析

Correlation of H-FABP Gene Expression with IMF and Fatty Acid Content in Laiwu Pigs and Duroc

旨在研究莱芜猪和杜洛克猪间肌肉H-FABP基因mRNA表达差异,同时分析H-FABP基因mRNA表达量与肌内脂肪和脂肪酸含量的相关性,探索莱芜猪肌内脂肪沉积的分子机理。试验选择100kg体重莱芜猪10头、杜洛克7头,采用荧光定量RT-PCR法测定H-FABP基因mRNA表达丰度,并测定肌内脂肪和脂肪酸含量。结果表明,莱芜猪背最长肌H-FABP基因mRNA的表达量比杜洛克猪高36.16%。莱芜猪和杜洛克猪肌肉H-FABP基因mRNA的表达量与肌内脂肪含量相关显著,H-FABP基因mRNA的表达量和肌内脂肪含量与饱和脂肪酸、饱和+单不饱和脂肪酸含量相关显著,莱芜猪H-FABP基因mRNA的表达量和肌内脂肪含量与多不饱和脂肪酸含量相关显著,与脂肪酸总量相关不显著,但是杜洛克猪则与此相反。莱芜猪和杜洛克猪肌肉H-FABP基因mRNA表达量与肌内脂肪和脂肪酸组成相关显著,该基因可作为莱芜猪肌内脂肪选育的候选基因。

This experiment was conducted to study the differential expression of H-FABP gene between Laiwu pigs and Duroc,and analysis correlation of H-FABP gene expression with IMF（intramuscular fat） and fatty acid con- tent of longissimus muscle. Ten Laiwu pigs and seven Duroc weighting approximately 100 kg were chosen for the experiment. Expression of H-FABP at mRNA in LM of Laiwu pigs and Duroc were measured by real time fluorescence RT-PCR,IMF and fatty acid content were measured. The result indicated that,Compared with Duroc,H-FABP gene expression of longissimus muscle in Laiwu pigs was increased by 36. 16% . Correlation between H-FABP gene expression and IMF content with SFA（saturated fatty acid） ,SFA ＋ MUFA （monounsaturated fatty acids） in Laiwu pigs Duroc was prominent. Correlation between H-FABP gene expression and IMF content with PUSA（polyunsaturated fatty acid） was distinctly negative,with sum of fatty acid in Laiwu pigs was not remarkable,that＇s of Duroc was contrary. Correlation between H-FABP gene expression with IMF content and fatty acid composing was marked,so H-FABP is candidate gene of IMF in Laiwu pigs＇ breeding.