猪流感病毒河南株的分离鉴定和生物学特性

Isolation,Identification and Biological Characterization of Swine Influenza Viruses in Henan

从河南省有呼吸道症状的5个不同猪场采集棉拭子和肺组织样品。样品处理后,通过尿囊腔途径接种10日龄SPF鸡胚。获得3株病毒,经血凝和血凝抑制试验鉴定,为H3N2亚型流感病毒,并对其部分生物学特性进行了研究。结果表明,其半数鸡胚感染量(EID50)分别是0-9.56/0.2mL,10-8.56/0.2mL和10-8.44/0.2mL,对小白鼠的半数致死量(LD50)分别是10-1.56/0.1mL,10-1.45/0.1mL和10-1.50/0.1mL;它们的血凝素对热不稳定,能凝集1%马、牛、驴、猪、绵羊、鸡、兔、豚鼠、小白鼠及人O型血的红细胞。这为了解河南猪群中流行的流感病毒奠定了基础。

Samples were collected by cotton swabs and lung tissues from pigs with respiratory system problems on 5 farms in Henan province. Samples were inoculated into 10-day-old SPF-embryonated eggs via the allantoic route,after being processed. Three isolates were obtained and identified as influenza A virus subtype H3N2 by HA and HI. Some biological properties of them were studied. 50% egg infective doses （EID50） were 10^ -9. 56/0. 2 mL,10 ^-8. 56/0. 2 mL and 10 ^-8. 44/0. 2 mL. 50% lethal doses （LD50） to small white mice of the strain were 10 ^-1. 56/0. 1 mL,10^ -1. 45/0. 1 mL and 10 ^-1. 50/0. 1 mL ,respectively. Hemagglutinins of the strains were unstable to heat. Red blood cells of horse, cattle,pig,donkey,pig,sheep,cock,rabbit,guinea pig,small white mouse,big white mouse and human O-type could be agglutinated by the isolate. It will lay a foundation for understanding influenza viruses circulating in swine herds in Henan province.