枯草芽孢杆菌对盐胁迫条件下黄瓜根际酶活性的影响

Effects of Bacillus subtilis on Rhizosphere Enzyme Activities of Cucumber under Salt-stress

通过基质栽培方式,研究了枯草芽孢杆菌在盐胁迫条件下对黄瓜根际脱氢酶、多酚氧化酶、过氧化氢酶、过氧化物酶、脲酶和碱性磷酸酶活性的影响。结果表明,在无盐胁迫条件下,枯草芽孢杆菌提高了根际脱氢酶、脲酶和碱性磷酸酶的活性,显著提高了根际多酚氧化酶和过氧化氢酶的活性;在低浓度盐胁迫,即2倍正常浓度营养液浇灌下,枯草芽孢杆菌提高了根际脱氢酶、过氧化物酶活性,显著提高了根际过氧化氢酶、脲酶和碱性磷酸酶的活性;在高浓度盐胁迫,即4倍浓度营养液浇灌下,添加枯草芽孢杆菌对根际酶活性的影响与不加菌处理没有显著差异。

Effects of Bacillus subtilis （B.S） on dehydrogenase activity,polyphenoloxidase activity,catalase activi- ty,peroxidase activity,urease activity and alkaline phosphatase activity of cucumber under salt-stress was studied through substrate culture. The results showed that under no salt stress,Bacillus subtilis increased dehydrogenase activity,urease activity and alkaline phosphatase activity,significantly increased polyphenoloxidase activity and catalase activity in the rhizosphere. Under lower-concentration salt stress,（i.e. double of normal nutrient solution level） ,Bacillus subtilis increased dehydrogenase activity and peroxidase activity,significantly increased catalase activity,urease activity and alkaline phosphatase activity in the rhizosphere. Under higher-concentration salt stress,（i.e. four times of normal nutrient solution level） ,compared to no B. S treatment,Bacillus subtilis had no significant influence on rhizosphere enzyme.