电离辐射联合自噬诱导剂或抑制剂对人宫颈癌Hela细胞的作用

The Effects of Ionizing Radiation Combined with Autophagy Inducers or Inhibitors on Human Cervical Cancer Hela Cells

目的探讨电离辐射联合自噬抑制剂或诱导剂对人宫颈癌细胞株增殖的影响。方法采用四甲基偶氮唑蓝(MTT)和流式细胞术(FCM)检测经不同剂量(0、2、4、6、8和10 Gy)照射和6 Gy+自噬抑制剂三甲基腺嘌呤(3-MA)及6Gy+自噬诱导剂雷帕霉素(rapamycin)不同方法处理的人宫颈癌细胞存活和增殖情况,并分析其量效和时效关系。结果随着照射剂量的增加(2、4、6、8和10 Gy)及时间的延长(24、48和72 h),宫颈癌细胞增殖的抑制作用组间比较差异有统计学意义(P<0.05或P<0.01)。6 Gy+rapamycin处理对癌细胞增殖的抑制率低于单纯6Gy照射组(P<0.05),组间比较差异有统计学意义(P<0.05或P<0.01)。6 Gy+3-MA处理对癌细胞增殖的抑制高于单纯6 Gy照射组(P<0.05),处理后24、48和72 h其抑制率组间比较差异有统计学意义(P<0.05或P<0.01)。结论在Hela细胞中电离辐射诱导自噬能够拮抗凋亡,电离辐射联合自噬诱导剂能够抑制Hela细胞凋亡,而电离辐射联合自噬抑制剂能够促进其凋亡。

Objective To detect the effects of ionizing radiation combined with autophagy inhibitors and inducers on the proliferation of human cervical cancer cell line.Methods MTT and flowcytometry（FCM） were used to detect the surviving and proliferation of human cervical cancer cells,and analysis of the relationship of dose-effect and time-effect was made.Results With the increase of irradiation doses（2,4,6,8 and 10 Gy） and the elongation of irradiation time（24,48 and 72 h）,the inhibiting effect of ionizing radiation on the proliferation of human cervical cancer cells increased（P 〈0.05 or P 〈0.01）.The inhibiting effect of 6 Gy combined with autophagy inducer rapamycin on the proliferation of Hela cells weakened（P 〈0.05）.The inhibiting effects of 6 Gy combined with autophagy inhibitor 3-MA on the cell proliferation were higher than those in 6 Gy group（P〈 0.05）.Conclusion Ionizing radiation combined with autophagy inducers can inhibit apoptosis in Hela cells,while the ionizing radiation combined with autophagy inhibitors can promote their apoptosis.