复方党参通气液对实验兔腹部术后血浆SP、MOT的影响

Effect of Dangshen Compound Peristalsis-Promoting Decoction on the Plasma Levels of Substance-P and Motilin in Rabbits After Abdominal Operation

目的:观察复方党参通气液对实验兔腹部手术后胃肠激素P物质(SP)、胃动素(MOT)水平的影响。方法:65只实验兔随机分为正常对照组(10只)、假手术组(10只)、模型组(45只)。模型组又分为实验组(复方党参通气液组)、对照组(大承气汤组)、空白组(生理盐水)。观察术后中药治疗对血浆中SP、MOT的影响。结果:假手术组术后第1d血浆中的SP、MOT水平较正常对照组有一定的下降(P<0.05,P<0.01),第7d血浆中SP、MOT水平基本恢复正常(P>0.05);空白组术后第1d和第7d血浆中的SP、MOT水平均明显降低,与假手术组比较有显著差异(P<0.05)。在药物治疗后7d,实验组血浆中SP、MOT水平与对照组及空白组比较均有明显差异(P<0.01,P<0.05)。结论:复方党参通气液能刺激兔胃肠激素SP、MOT的分泌增多,促进胃肠功能恢复。

Objective To study the effect of Dangshen Compound Decoction on the plasma levels of Substance-P（SP）and Motilin（MOT）in rabbits after abdominal operation.Methods Rabbit abdominal operation models were prepared with segmental resection of 2cm colon under general anaesthesia（n=45）.Each group（fifteen models）were treated with gavage of ① Danshen Compound Decoction ② Da Cheng Qi decoction（another chinese traditional preparation for peristalsis-promoting）and ③ saline respectively for 6 days.Another 10 rabbits underwent sham operation（anaesthesia and laparatomy only）.With no gavage.Plasma levels of SP and MOT were determined with RIA in all these animals on d1 and d7 after operation and once in another 10 untouched rabbits.Results In the animals with sham operation,the plasma SP and MOT levels dropped on d1 after operation and were significantly lower（P〈0.05,P〈0.01）than those in untouched animals,but recovered to approaching normal on d7（vs untouched animals,P〉0.05）.In the operation models with saline gavage only,the plasma levels on d1 and d7 were all significantly loer than the corresponding ones in the sham group（P〈0.05）.In the models treated with Dangshen Compound Decoction,the plasma SP and MOT levels on d7 postoperatively were significantly higher than those in models treated with either Da Cheng Qi Decoction or saline（P〈0.01,P〈0.05）,being even a little higher than those in untouched animals.Conclusion Dangshen Compound Decoction can effectively stimulate the secretion of substance-P and motilin,thus promoting recovery of peristalsis in rabbits after abdominal operation（colon segmental resection）.