摘要
目的:探讨遗传背景对心房颤动(atrial fibrillation,AF)发生、发展的影响。方法:房颤组121例、对照组100例,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法鉴定AT1R基因1166位点基因型并对房颤组做彩色超声心动图进行比较,Logistic回归分析基因型对房颤的独立危险性。结果:房颤组AC+CC基因型频率、C等位基因频率均较对照组增高(P分别为0.017、0.013),与AA型相比,AC型+CC型者发生AF的风险率为3.657(95%CI:1.181-11.322),基因型、收缩压参与增加总体AF发生的概率(P分别为0.019、0.002),以AA型为参照,AC型+CC型发生AF的OR为4.132(95%CI:1.263-13.513)。②AF患者有无心衰、脑栓塞并发症病史基因型频率及等位基因频率比较差异无显著性(P〉0.05),AF患者心脏超声指标AA型者与AC型+CC型者比较差异无显著性(P〉0.05)。结论:携带AT1R基因1166位点C等位基因者有增加发生AF风险的倾向,但与病程发展中心脏结构、功能改变以及心力衰竭、脑栓塞等并发症无显著性关系。
Objective To investigate the effect of genetic polymorphism on atrial fibrillation.Methods Polymerase chain reaction-restrictive fragment length polymorphism(PCR-RFLP)was used to identify and compare the genotype of the location of AT1R gene 1166,and color echo-ultrasound was performed with logistic regression used to analyse the independent risk of various genotypes for atrial fibrillation in 121 patients with atrial fibrillation and 100 controls.Results ① Frequency of genotype AC+CC,isogene C in atrial fibrillation group was higher than that in control group(P=0.017,0.013),the risk ratio in patients with genotype AC+CC to develop atrial fibrillation was 3.657 compared with genotype AA(95% CI:1.181-11.322),and genotype difference as well as systolic pressure were involved in ocurrence of overall atrial fibrillation.The OR to develop atrial fibrillation in patients with genotype AC+CC was 4.132 compared with genotype AA(95% CI:1.263-13.513).② There were no significant differences of clinical manifestation(heart failure,cerebral embolism)or ultrasonic parameters among patients with different genotypes(AA vs AC+CC)(P〉0.05).Conclusion People carrying isogene C in AT1R gene 1166 were more liable to develop atrial fibrillation,but there were no correlationship with development of complications.
出处
《放射免疫学杂志》
CAS
2010年第4期421-424,共4页
Journal of Radioimmanology
基金
广东省医学科研项目(A2007613)
珠海市科技计划项目(PC20071008)