血管紧张素Ⅱ-1型受体基因A1166C多态性与心房颤动并发症相关性研究

Study of Correlation Between Polymorphism of Angiotensin Ⅱ-1 Receptor Gene A1166 Genotype and Complications in Atrial Fibrillation

目的：探讨遗传背景对心房颤动（atrial fibrillation,AF）发生、发展的影响。方法：房颤组121例、对照组100例,聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法鉴定AT1R基因1166位点基因型并对房颤组做彩色超声心动图进行比较,Logistic回归分析基因型对房颤的独立危险性。结果：房颤组AC＋CC基因型频率、C等位基因频率均较对照组增高（P分别为0.017、0.013）,与AA型相比,AC型＋CC型者发生AF的风险率为3.657（95%CI：1.181-11.322）,基因型、收缩压参与增加总体AF发生的概率（P分别为0.019、0.002）,以AA型为参照,AC型＋CC型发生AF的OR为4.132（95%CI：1.263-13.513）。②AF患者有无心衰、脑栓塞并发症病史基因型频率及等位基因频率比较差异无显著性（P〉0.05）,AF患者心脏超声指标AA型者与AC型＋CC型者比较差异无显著性（P〉0.05）。结论：携带AT1R基因1166位点C等位基因者有增加发生AF风险的倾向,但与病程发展中心脏结构、功能改变以及心力衰竭、脑栓塞等并发症无显著性关系。

Objective To investigate the effect of genetic polymorphism on atrial fibrillation.Methods Polymerase chain reaction-restrictive fragment length polymorphism（PCR-RFLP）was used to identify and compare the genotype of the location of AT1R gene 1166,and color echo-ultrasound was performed with logistic regression used to analyse the independent risk of various genotypes for atrial fibrillation in 121 patients with atrial fibrillation and 100 controls.Results ① Frequency of genotype AC＋CC,isogene C in atrial fibrillation group was higher than that in control group（P=0.017,0.013）,the risk ratio in patients with genotype AC＋CC to develop atrial fibrillation was 3.657 compared with genotype AA（95% CI：1.181-11.322）,and genotype difference as well as systolic pressure were involved in ocurrence of overall atrial fibrillation.The OR to develop atrial fibrillation in patients with genotype AC＋CC was 4.132 compared with genotype AA（95% CI：1.263-13.513）.② There were no significant differences of clinical manifestation（heart failure,cerebral embolism）or ultrasonic parameters among patients with different genotypes（AA vs AC＋CC）（P〉0.05）.Conclusion People carrying isogene C in AT1R gene 1166 were more liable to develop atrial fibrillation,but there were no correlationship with development of complications.