L-谷氨酰胺对次声致大鼠记忆障碍的防护效应及部分机制

Protective effects of L-glutamine on infrasound-induced dysmnesia in rats and its partial mechanism

目的探讨L-谷氨酰胺(L-Gln)对次声暴露下大鼠记忆能力、脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。方法将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组、药物→次声组,分别将次声组、次声+药物组、药物→次声组暴露于16Hz、130dB次声环境中,2h/d,正常对照组也置于次声舱内,但期间不给予次声作用。经7d相应处理后,测试大鼠寻找到平台的潜伏期、SOD活性、MDA含量的变化。结果与正常对照组比较,次声组寻找到平台的潜伏期明显延长(P<0.01),SOD活性、MDA含量明显升高(P<0.05或P<0.01);次声+药物组、药物→次声组与次声组比较,潜伏期明显缩短(P<0.05),SOD活性升高、MDA含量降低(P<0.05或P<0.01)。结论 L-Gln能够改善大鼠次声性脑损伤的记忆障碍,部分机制与其抗氧化损伤有关。

Objective To explore the effects of L - glutamine （ L - Gln） on memory ability, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in brains of rats exposed to 16 Hz/130 dB infrasound. Methods 60 male SD rats were randomly divided into four groups： normal control group, infrasound group （ 16 Hz/130 dB infrasound） , infrasound ＋ drug group （16 Hz/130 dB infrasound ＋ L - Gin）,drug→infrasound group （L -Gln for 7 d,and then exposed to 16 Hz/130 dB infrasound）. Animals in normal control group were placed in an infrasound chamber but not exposed to infrasound, while other three groups were exposed to 2 h of infrasound in the same chamber everyday. 7 d later, the latency of rats to find the platform in Morris water maze test was examined as the indicator of memory ability. SOD activity and MDA content in the brain tissue was also detected. Results Infrasound group showed significant longer latency to find the platform, higher SOD activity and higher MDA content than normal control group did （P 〈0.05 or P 〈0. O1 ）. Compared with infrasound group,infrasound ＋ drug group and drug→infrasound group exhibited significantly shorter latency to find the platform ,higher SOD activity and lower MDA content （P 〈 0.05 or P 〈 0.01 ） . Conclusion L - Gin can improve the dysmnesia caused by brain subaudible injury in rats partially due to its anti - oxidative effects.