直肠癌组织中膜联蛋白A1和A2表达水平及临床病理意义

Expression of Annexin A1 and A2 in Rectal Cancer Tissues and Its Clinicopathological Significance

[目的]研究直肠癌及其癌旁组织中膜联蛋白A1（ANXA1）和A2（ANXA2）表达水平及其临床病理意义.[方法]50例直肠癌和20例癌旁组织手术切除标本常规制作石蜡包埋切片,ANXA1和ANXA2染色方法为EnVisionTM二步免疫组化法.[结果]直肠腺癌ANXA1和ANXA2表达阳性率明显地高于癌旁组织（P＜0.01和P＜0.05）,ANXA1和（或）ANXA2表达阳性的癌旁组织均呈中至重度不典型增生;高分化腺癌、癌细胞未侵犯浆膜层、淋巴结未转移及Dukes分期A＋B期病例ANXA1和ANXA2表达阳性率明显低于中＋低分化腺癌,癌细胞侵犯浆膜层,淋巴结转移及Dukes分期C＋D期病例（P＜0.05）;直肠癌中ANXA1和ANXA2表达呈高度一致性（χ^2=9.43,P＜0.01）.[结论]ANXA1和（或）ANXA2表达水平可能是反映直肠癌发生、进展、生物学行为及预后的重要生物学标记物.

[Objective] To study the expression of annexin A1 （ANXA1） and A2（ANXA2） in rectal cancer and adjacent non-tumor tissues, and explore its clinicopathological significance. [Methods] The surgical specimens of 50 cases of rectal cancer and 20 adjacent non-tumor tissues were routinely prepared to be paraffin-embedded sections. EnVisionTM immunohistochemistry method was used to detect the expression of ANXA1 and ANXA2. [Results]The positive rates of ANXA1 and ANXA2 were significantly higher in rectal cancer than those in adjacent non-tumor tissues（ P 〈0.01, P 〈0.05）. The positive cases of ANXA1 and/or ANXA2 in adjacent non-tumor tissues showed moderate to severe atypical hyperplasia. The positive rates of ANXA1 and ANXA2 were significantly lower in the well differentiated adenocarcinoma and tissues without invasiveness of serous layer and lymph node metastasis or with Dukes stage A＋B than those in the moderately and poorly-dif ferentiated adenocarcinoma and tissues with the invasiveness of serous layer, metastasis of lymph node or Dukes stage C＋D（ P 〈0. 05）. The high consistence was found between the expression of ANXA1 and ANXA2 in rectal cancer tissues （χ^2 =9.43, P〈0.01）. [Conclusion]The expressive levels of ANXA1 and/or ANXA2 may be important biological markers for reflecting the carcinogenesis, progression, biological behaviors and prognosis of rectal cancer.