肝癌发生、发展过程中血管生成素-2的动态表达与改变

Dynamic expression and alteration of angiopoietin-2 during the occurrence and development of hepatocellular carcinoma

目的:观察肝癌发生发展过程中血管生成素-2(Ang-2)的动态改变及其与血管内皮生长因子(VEGF)表达的关系。方法:以2-乙酰氨基芴诱发雄性SD大鼠肝细胞癌变,观察癌变过程中肝组织的病理学变化,以免疫组织化学法或酶联免疫吸附法(ELISA)观察Ang-2和VEGF动态表达及相互关系。结果:诱癌过程中鼠肝细胞出现颗粒样变性、不典型增生、癌前病变及肝癌形成的动态变化;血Ang-2水平,在正常对照组为105.23±19.23ng/L、肝细胞变性组为140.17±25.16ng/L、癌前病变组为203.75±23.83ng/L和癌变组为222.39±23.31ng/L,随着病理组织学的改变呈梯度增加,肝癌组明显高于对照组和变性组(P<0.001);癌变过程中Ang-2与VEGF的动态改变呈显著正相关(r=0.785,t=8.00,P=0.000)。结论:肝细胞癌变过程中Ang-2及VEGF呈过表达状态,肝癌的发生发展与新血管生成密切相关。

Objective：To investigate the dynamic expression of hepatic angiopoietin-2（Ang-2） and association with vascular endothelial growth factor（VEGF） during the occurrence and development of hepatocellular carcinoma（HCC）.Methods：The dynamic changes of rat liver pathology were observed through the hepatoma model.The expression of circulating Ang-2 and VEGF was analyzed by ELISA or immohistochemistry and their relationship was explored.Results：The hepatic Ang-2 and VEGF expression inereased progressively during the HCC development course from normal hepatocytes to the denatured hepatocytes to precancerous to the formation of HCC.The levels of circulating Ang-2 expression were 105.23±19.23ng/L in the control group,140.17±25.16ng/L in the denatured group,203.75±23.83ng/L in the precancerous group,and 222.39±23.31ng/L in the cancerous group,respectively.There were significant differences between the control group and any of the experimental groups（P〈0.001） and closely positive relationship between Ang-2 and VEGF（r=0.785,t=8.00,P〈0.001）.Conclusions：The expressions of hepatic Ang-2 and VEGF are overexpressed,and the occurrence and development of HCC are associated with new angiogenesis.