莫诺苷对局灶性脑缺血再灌注大鼠Caspase-3活化程度的影响

Effects of Morroniside on Activation of Caspase-3 in Rats with Focal Cerebral Ischemia/Reperfusion

目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠海马caspase-3活化程度的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,缺血30min,再灌注72h。以维生素E(VE)为阳性对照药,采用分光光度法检测脑海马组织caspase-3活性变化。结果与假手术组相比,模型组caspase-3活性明显增加(P<0.001);与模型组相比,莫诺苷30mg/kg、90mg/kg、270mg/kg均能够降低caspase-3活性,并呈剂量依赖性(P<0.05);VE(35mg/kg)能显著降低caspase-3活性(P<0.001)。结论莫诺苷能够抑制大鼠脑组织caspase-3活性,通过抗凋亡发挥神经保护作用。

Objective To investigate the effects of morroniside on activation of caspase-3 in rats with focal cerebral ischemia/reperfusion.MethodsThe model was induced with occlusion of middle cerebral artery with suture embolus,ischemia for 30 min,and reperfusion for 72 h in rats.Vitamin E used for the positive control.The activity of caspase-3 was detected with spectrophotometry.ResultsCompared with sham group,the caspase-3 activity increased obviously in model rat.Compared with model group,Morroniside（30 mg/kg,90 mg/kg,270 mg/kg）decreased the activation of caspase-3 remarkably,which was dose-dependent（P〈0.05）.ConclusionMorroniside may reduce apoptosis by decreasing the activation of caspase-3 in rats.