不同种源黄连木遗传多样性研究

Genetic Diversity of Different Geographical Populations of Pistacia chinesis Bunge

采用RAMP对我国不同地区的180份黄连木材料进行遗传多样性、种群内和种群间的遗传变异进行了研究。结果表明,13对引物组合所产生的115条DNA扩增片段中,有109条具有多态性,多态位点百分率PPB为94.78%;Shannon多样性指数I=0.5541,Nei's多样性指数H=0.38030,物种总基因多样性(Ht)为0.2963;种群内基因多样性(Hs)为0.3803,群体间基因分化系数(Gst)为0.2209,表明22.09%的变异存在于种群间,群体内的变异占总变异的77.91%;基因流(Nm)为1.7633,显示种群间的基因交流顺畅。

The genetic diversity of Pistacia chinesis Bunge.was analyzed using random amplified microsatellite polymorphism（RAMP） markers.The results indicated that there was relatively high genetic diversitv in China.115 bands were amplified from 6 populations in different habitats with 13 reliable primers,109 bands were polymorphic,The polymorphic percentage was 94.78%;The Shannon information index I was 0.554 1 and Nei＇s index H was 0.380 30.The total gene diversity Ht was 0.296 3,and the gene diversity within populations Hs was 0.380 3. The Gst was 0.220 9,77.91%of genetic variation existed within populations while 22.09%of genetic variation existed among populations,and the gene flow was 1.763 3.There was a strong gene flow among the populations,So the genetic drift would not cause the genetic differentiation.