菊花滑刃线虫快速分子检测

A Rapid Method to Detect Aphelenchoides ritzemabosi by PCR

根据菊花滑刃线虫(Aphelenchoides ritzemabosi)和相近滑刃线虫种群的rDNA-ITS序列,设计出菊花滑刃线虫特异性引物,利用PCR技术对菊花滑刃线虫包含部分5.8S基因和部分rDNA-ITS2核苷酸序列进行扩增,获得208bp的特异性片段。实现了单条活的或FG(4%的甲醛)固定的菊花滑刃线虫的快速检测。

A polymerase chain reaction （PCR） was used to amplify partial ribosomal DNA containing 5.8S gene and partial internal transcribed spacer region 2（ITS2） .According to the difference between the sequences of internal transcribed spacer region （ITS） of Aphelenchoides ritzemabosi,A.besseyi and that of A.fragariae,specific primers were designed respectively for A.ritzemabosi.The primers amplified a fragment of about 208 bp.A single nematode,living or preserved in formalin,could be detected rapidly,and A.ritzemabosi was distinguished as well.This method would be useful for rapid detection of nematodes.