细胞凋亡与妊娠期肝内胆汁淤积症妊娠结局的关系

The relationship between gene apoptosis and the pregnancy outcome of ICP

目的:研究妊娠期肝内胆汁淤积症(ICP)患者胎盘细胞凋亡与羊水污染、新生儿窒息、早产、剖宫产的关系,探讨ICP患者妊娠结局不良的原因。方法:采用免疫组织化学方法检测ICP组和正常组凋亡相关基因Caspase-3的表达水平,再根据Caspase-3表达强弱将ICP组孕妇分为3组,了解羊水污染和新生儿窒息的发生率,探讨ICP患者胎儿预后不良是否与胎盘细胞凋亡有关。结果:ICP组胎盘细胞促细胞凋亡相关基因Caspase-3表达增加,且随着促凋亡基因表达的增加临床上表现为羊水污染及剖宫产几率升高。结论:ICP患者胎盘细胞促凋亡基因表达增加,胎盘细胞过度凋亡,与血清胆酸水平有相关性,使胎盘功能减退,导致羊水污染的几率增加。

Objective：To investigate the relationship between apoptosis and neonatal asphyxia,amniotic fluid pollution,premature labor,cesarean section among ICP women,and discuss the reason for bad results of pregnant ICP women.Methods：Immuno-histochemistry methods were preformed to study the expression of Caspase-3 from ICP women and normal women.According to expression strength of Caspase-3,the ICP women were divided into 3 groups,and check the ratio of amniotic fluid pollution and asphyxia neonatorum.Results：The expression of Caspase-3 in ICP women was higher than that in normal women.In this case,amniotic fluid pollution and cesarean section were also higher than normal.Conclusion：The apoptosis gene in the placenta of ICP women increases,and too much apoptosis,are related with serum cholalic acid.All these factors can cause placenta hypofunction and amniotic Fluid pollution.