在位与异位子宫内膜细胞凋亡及免疫组化研究

Research on the apoptosis and proliferation features in ectopic endometrial cells of uterus and ovary

目的:探讨子宫腺肌病与卵巢内膜异位囊肿异位内膜细胞凋亡和增殖特性及发病机制。方法:2004年6月～2008年6月手术病例采用免疫组化EnVision法检测子宫腺肌病和卵巢异位囊肿各60例的在位及异位子宫内膜中凋亡调控基因蛋白Bcl-2、bax及细胞增殖标记物ki-67蛋白表达。结果:Bcl-2蛋白、bax蛋白及ki-67蛋白在两病的在位内膜及子宫腺肌病异位内膜中均呈现周期性改变,而Bcl-2蛋白及ki-67蛋白在卵巢异位囊肿异位内膜中呈持续性增强,较在位内膜差异有统计学意义(P<0.05)。结论:子宫腺肌病异位内膜的细胞凋亡和增殖受卵巢性激素周期性调节,卵巢异位囊肿异位内膜则相反,两者细胞凋亡和增殖特性截然不同,是两种不同的疾病。

Objective：To investigate the property of apoptosis and proliferation of the ectopic endometrial cells in adenonyosis and ovarian ectopic cyst as well as their possible pathogenesis.Methods：To detect the expression of the apoptotic protein Bcl-2,bax and the cell proliferating labeling antigen ki-67 in eutopic and ectopic endometrial cells of adenomyosis and ovarian ectopic cyst with the immunohistochemical s-p method.Results：Protein Bcl-2,bax and ki-67 cyclically changed both in the eutopic and the ectopic endometrial cells of adenomyosis and ovarian cyst; while protein Bcl-2,bax and ki-67 of ovarian ectopic cyst were continuously enhanced,which was significantly different from that of eutopic endometrium（P〈0.05）.Conclusion：The apoptosis and proliferation of the ectopic endometrial cells in adenomyosis are cyclically adjusted by ovary,while those in ovarian ectopic cyst are contrary to them,either of which has its own origin.