抗蛋白吸附PLLA-MPEG微绒毛膜的制备

Preparation of PLLA-MPEG Microbrushes for Resistance of Protein Adsorption

在左旋聚乳酸(PLLA)膜上采用朗格缪尔-布洛杰特(LB)技术、浇铸和化学接枝的方法制备了左旋聚乳酸-甲基端聚乙二醇(PLLA-MPEG)共聚物微绒毛膜。通过原子力显微镜(AFM)和水接触角测量等方法考察了制备方法及条件对绒毛层性能的影响,对绒毛层的亲水性、稳定性和抗蛋白吸附性能进行了对比分析。结果表明:通过这3种方法均能获得较亲水的抗蛋白吸附表面。PLLA-MPEG单分子LB微绒毛必须在热水中处理后才能牢固地黏附于PLLA膜上;浇铸的PLLA-MPEG成膜性好,但遇水易脱落,稳定性差;紫外光催化接枝的微绒毛制备简便、铆接牢固,可用于医疗器械或生物埋置材料的表面修饰。

Poly（L-lactic acid）-co-poly（ethylene glycol）（PLLA-MPEG） microbrushes were prepared by LB technique,casting and chemical grafting onto PLLA films.The effects of preparing conditions on the pro-（perties） of microbrush layers were studied by atomic force microscope（AFM） and water contact angle.The stability,hydrophilicity and protein resistance ability of these microbrush layers were also thoroughly studied and compared.Results show that PLLA-MPEG microbrush layers prepared by the three methods are all hydrophilic and show resistance of protein adsorption.PLLA-MPEG LB film is dealt with hot water to（increase） its stability.The casting PLLA-MPEG layer has a good filming property but the stability is bad.The microbrushes prepared by photocatalysis method could bind firmly on PLLA casting film,which is possible to be used in surface modification of medical devices and implanting materials.