VEGFR2低表达的CRL2830细胞体外模型的建立和生物学特性研究

Establishment of CRL2830 Cell Model in Vitro with VEGFR2 Low Expression and Study of Its Biological Characteristics

采用3-D培养和shRNA技术首次建立了VEGFR2低表达的人源性多囊肾CRL2830细胞体外模型,进一步考察该基因表达抑制对多囊肾细胞生物学特性的影响.研究结果表明:与亲本细胞相比,VEGFR2表达抑制虽然不影响细胞生长速度和侵袭能力,但能引起细胞形态明显改变,细胞和细胞核的体积增大,细胞容易形成团状结构;3-D培养结果表明VEGFR2低表达能显著延缓囊肿的生长速度,有效诱导囊肿细胞早期凋亡.

An idea model of polycystic kidney cells CRL2830 with angiogenesls tactor receptor 2 （VEG- FR2） low expression was established by applying 3-D culture and shRNA technology, which can be applied to reveal the role of VEGFR2 in polycystic kidney disease. The change of biological characteristics in this cell line was observed,and the results have shown that, although VEGFR2 low expression does not affect cell growth and invasion, it can result in the volume increase of cell and nucleus and the enhancement of cell aggregation. Furthermore, the low expression of VEGFR2 can not only inhibit cyst growth but also efficiently induce early cell apoptosis in 3-D culture cells, compared with parental cells.