结直肠癌细胞辐射敏感性的定量比较蛋白组学研究

Quantitative and Comparative Proteomics Analysis of Radiosensitivity for Colorectal Cancer Cells

为筛选结直肠癌细胞辐射敏感相关的蛋白质,用于结直肠癌放疗的个体化选择,采用荧光差异显微凝胶电泳(DIGE)技术分离并筛选Cy3、Cy5及Cy2荧光素标记的不同辐射敏感性的结直肠癌细胞系SW480和LOVO的移植瘤组织的差异表达蛋白质,结果共筛选出35个差异表达蛋白点,其中,在SW480组肿瘤组织中表达上调的蛋白点有17个,在LOVO组肿瘤组织中表达上调的蛋白点有18个。用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术进行鉴定并分析,成功鉴定蛋白质点27个,LOVO组肿瘤组织表达上调蛋白17个,SW480组肿瘤组织表达上调蛋白15个。这些差异表达蛋白质有可能在结直肠癌辐射敏感性的预测中作为特异性的标记物,在结直肠癌患者的放疗选择中发挥重要作用,为结直肠癌患者的治疗选择提供技术参考。

To identify the related proteins about radiosensitivity（RS）of colorectal cancers,as well as make the personal therapeutics for patients,the specimen of transplanted tumor raised by the colorectal cancer cells,LOVO and SW480with different radiosensityvity,were examined by the fluorescence differential in-gel electrophoresis（DIGE）after labeled with CyDye DIGE fluors Cy3,Cy5and Cy2.As the result,intensity changes of 35spots were detected with statistical significance.18protein spots of them are upregulated in tumor specimen of LOVO,otherwise 17are up-regulated in specimen of SW480.And 27of them were identified by MALDI-TOF-MS successfully including 17 in LOVO and 15in SW480.These differential expressed proteins may become the mocu-lar markers and have a key role in predicting the RS of colorectal cancer.