特殊广亲和系GC13的SSR聚类及与杂种育性相关位点分析

SSR Cluster of Special Wide Compatibility Line GC13 and Its Fertility Loci

为了阐明GC13的特殊广亲和性遗传机理,从分子水平上分析GC13的籼粳聚类,通过用70对SSR引物对GC13及5个籼稻、5个粳稻和5个广亲和品种进行聚类分析。结果表明,GC13归入粳稻类型,与籼型品种及广亲和品种的亲缘关系较远,并在GC13中定位到两个与育性相关的位点,其一与第6染色体上RM225紧密连锁,根据所在染色体位置,推测此位点与S5等位,且GC13在此位点上的等位基因为S5-n;其二与第8染色体上RM408连锁,暂命名为Sg(t),GC13在此位点上携带Sg(t)-i等位基因,与IR36一致。S5位点的效应大于Sg(t)。

Japonica line GC13 was considered as a special wide compatibility variety（SWCV）,because its compatibility was weak within subspecies and strong between subspecies.GC13 was clustered into japonica group by cluster analysis of GC13,5 indica varieties,5 japonica varieties and 5 wide compatibility varieties using 70 SSR markers.Bulked segregant analysis was performed to detect the fertility loci in the three-way cross of GC13/ Akihikari //IR36.Two SSR markers were detected to tightly link to fertility loci.One located linked closely to RM225 on chromosome 6 and mapped to S5 according to its chromosome location.GC13 carried neutral alleles S5-n in this locus.The other linked closely to RM408 on chromosome 8 and was tentatively designated as Sg（t）.GC13 carried allele Sg（t）-i as in IR36.The effect of S5 was larger than Sg（t）.The distribution of alleles at both loci S5 and Sg（t） can explain the mechanism of special wide compatibility in GC13,which will give useful information when GC13 was used in inter-subspecies hybrid rice breeding.