利用多重PCR技术鉴定玉米品种的初步研究

Preliminary Study of Maize Varieties Identification by Multiplex PCR Technology

在SSR分子标记的基础上,以3个玉米自交系、4个玉米杂交种和7对玉米SSR引物为材料,确定了较为适宜的多重PCR扩增体系和程序,获得了适宜琼脂糖凝胶电泳的、扩增效果较好的2～5重引物组合,并能有效应用于玉米杂交种和自交系的种子鉴定,不仅比单个引物鉴定更准确可靠,而且省时省工,成倍地降低了检测成本,将有利于推动多重PCR技术在玉米品种鉴定中的广泛应用。

In this research amplification system and procedure of multiplex PCR in Maize were established on the base of SSR molecular markers.Three maize inbred lines,four maize hybrids and seven maize SSR primers were used to obtain the combinations of 2-5 pairs of primers,which could produce a better amplified effect by agarose gel electrophoresis,and the combinations could be used in identification of maize hybrids and inbred lines effectively.Compared with a single primer,it was not only more accurate and reliable,but also saving the time and labor,keeping down the cost of the detection production.It would be conducive to promoting multiplex PCR technology in the identification of maize varieties in a wide range of applications.