西施舌鳃蛋白质组学研究

Study on the Gill Proteome of Coelomactra antiquate

[目的]研究西施舌（Coelomactra antiquate）鳃蛋白质组学。[方法]以西施舌的鳃组织为研究对象,经破碎、裂解和离心等抽提方法提取鳃组织蛋白质样品,利用Bradford蛋白质定量试剂盒作蛋白定量后,进行一向等电聚焦电泳和SDS聚丙烯酰胺凝胶电泳。电泳后的凝胶用考马斯亮蓝染色,利用Image ScannerⅢ扫描仪扫描凝胶,获得凝胶图像。[结果]经研究首次得到西施舌鳃蛋白质双向电泳图谱,初步构建了西施舌鳃蛋白质组的双向电泳技术体系。在pH值为4~7的范围内分布着大部的蛋白质,且多为酸性蛋白。其中,在低pH值区蛋白质分布较多,甚至还出现了高丰度蛋白质,在同一水平线上的蛋白质为分子量相近的蛋白质。[结论]该研究可为进一步探索西施舌鳃组织的生理生化机制奠定基础。

[Objective] The research aimed to study the gill tissue of Coelomactra antiquata.[Method] The gill tissue of Coelomactra antiquata was used for experimental material in this study.The protein samples of gill tissue of Coelomactra antiquate were extracted by crushing,cracking,centrifugation and other extraction methods.The Bradford protein quantitative kit was used for protein quantification.IEF electrophoresis and SDS-PAGE electrophoresis were carried out.The obtained gel was stained by coomassie brilliant blue R350,and the protein profile was scanned using Image Scanner Ⅲ scanner to obtain the gel image.[Result] It was the first time to get the two-dimensional gel electrophoresis profile and initially construct the two-dimensional gel electrophoresis system on the gill proteome of Coelomactra antiquate.The map analysis showed that the protein of Coelomactra antiquate gill mainly distributed in the range of pH value 4-7,and many were acidic protein.Thereinto,many proteins were distributed in region of low pH value,even also presented the high abundance protein.The protein on the same horizontal line was the same molecular weight protein.[Conclusion] This study can provide a foundation for the further exploration about the physiological and biochemical mechanisms on the gill proteome of Coelomactra antiquate.