精浆内抗精子抗体和免疫复合物的研究——对男性不育患者及输精管粘堵术者的观察

ANTISPERM ANTIBODIES AND IMMUNE COMPLEXES IN SEMINAL PLASMA OF IMFERTILE AND VAS-OCCLUDED MEN

本文用ELISA法研究了174名男性不育患者及43名输精管粘堵术后精浆中的抗精子抗体及其种类。结果表明,精浆中抗精子抗体的检出率,在男性不育患者中为24.1%,输精管粘堵术后者为48.8%。在这些患者的精浆中可检出IgA、IgG和IgM型抗精子抗体,其中主要是IgA。同组人群血清和精浆中的抗精子抗体缺乏相关性。精浆中抗精子抗体的存在与精子活动率的下降有一定的关系。应用PEG法测定精浆中免疫复合物,在66例精子抗体阳性的患者中,阳性率为56.1%,表明局部抗精子的自身免疫反应,在一定条件下可导致免疫复合物的形成。

Antisperm antibodies and their subtypes in seminal plasma of 174 infertile and 43 vas-occluded men were determined by enzyme-linked immunosorbent assay (ELISA). 24.1%of infertile and 48.8% of vas-occluded patients had positive reactions. IgA. IgG or IgMwere present in both groups, and IgA was majority of antibody species. No significantcorrelationship between antibody levels of seminal plasma and sera was shown in thesame groups. The patients with high level of seminal plasma antisperm antibodies would alsoget decreased sperm motility. Seminal plasma immune complexes measured with polyethy-lene glycol (PEG) method were found in 56.1% of patients with positive antisperm an-tibodies. These results indicate that antisperm antibodies and immune complex in seminalplasma may play important roles in etiology and pathogenesis of these diseases.