摘要
目的:研究"内关"穴位埋针对心肌缺血损伤小型猪心肌转化生长因子(TGF)-β3 mRNA和蛋白表达的影响,探讨穴位埋针抗心肌缺血的可能机制。方法:32只小型猪随机分为假手术组、模型组、内关组和膈俞组,采用左冠状动脉前降支结扎建立心肌缺血模型,内关组和膈俞组分别进行"内关""膈俞"穴埋针治疗,用realtime PCR和Western blot方法检测TGF-β3 mRNA和蛋白表达水平。结果:假手术组有少量TGF-β3 mRNA和蛋白表达,模型组TGF-β3 mRNA和蛋白表达量比假手术组升高(P<0.05),"内关"及"膈俞"穴位埋针治疗可上调TGF-β3 mRNA和蛋白表达量,与模型组比较差异有统计学意义(P<0.01),且在上调TGF-β3 mRNA和蛋白表达量方面"内关"优于"膈俞"(P<0.05)。结论:"内关"穴位埋针可能通过上调TGF-β3 mRNA和蛋白表达,刺激内皮细胞增殖,促进侧支循环建立,增加缺血心肌血流,实现对心肌组织的保护作用。
Objective To observe the effect of needle-implantation （NI） at ＂Neiguan＂ （PC 6） on the expression of myocardial transforming growth factor-beta 3 Protein（TGF-β3） and mRNA in Chinese miniswine with myocardial ischemia （MI） injury. Methods A total of 32 Chinese Guizhou miniswine were randomly and equally divided into Sham-operation group, model group, NI-PC 6 group and NI-Geshu （BL17, NI-BL17） group. MI injury model was established by occlusion of the descending ante- rior branch of the left coronary artery. Two acupuncture needles （veterinary use） were separately and subcutaneously implanted into ＂Neiguan＂ （PC 6） and ＂Geshu＂ （BLT7） areas for 7 days. TGF-β3 protein and mRNA expressions were determined by West- ern blot and real-time PCR techniques, separately. Results In comparison with the sham-operation （sham） group, TGF-β3 protein and mRNA expressions in model group were upregulated significantly（P〈0.05）. While compared with the model group, myocardial TGF-β3 mRNA expression was upregulated considerably in NI-PC 6 group （ P〈0.01 ）, rather than in NI-BL17 group （P〈0.05）, and myocardial TGF-β3 expression in both NI-PC 6 and NI-BL17 groups was upregulated obviously （P〈0. 01 ）. Comparison between NI-PC 6 and NI-BL17 groups showed that the expression levels of myocardial TGF-β3 protein and mRNA were significantly higher in NI-PC 6 group than in NI-BL17 group （P〈0.05）. Conclusion Needle-implantation of ＂Neiguan＂ （PC 6） can upregulate myocardial TGF-β3 protein and mRNA expression in MI Chinese miniswine, which may contribute to its effect in im- proving the ischemic myocardial injury by way of enhancing the angiopoiesis.
出处
《针刺研究》
CAS
CSCD
北大核心
2010年第4期267-271,共5页
Acupuncture Research
基金
贵州省长基金[黔省专合字2005(309)]
贵州省科技厅资助[黔科通2005(85)]
