家兔胆囊炎模型腹部循胆经高温反应与瘦素含量变化的观察

Observation on the Leptin Level in the Subcutaneous Tissues of the High Temperature Reaction Zone along the Abdominal Gallbladder Meridian in Rabbits with Cholecystitis

目的:研究兔胆囊炎模型腹部循胆经高温反应与瘦素含量的相关性。方法:实验用健康家兔分为正常对照组10只,胆囊炎模型组15只。胆总管缩窄结扎,于胆囊内注射大肠杆菌液0.1mL制作胆囊炎模型。造模后第3、7、11天分别沿胆经腹段,采用AGA-782型红外热像仪系统做体表热像图观察和记录。切取模型组循经高温反应部位皮下组织和对照组相应部位的组织,采用放射免疫法检测上清液内瘦素含量,并进行组织切片甲苯胺蓝染色的形态学观察。结果:体表红外检测结果显示模型组约有70%的动物在左右腹侧沿胆经的位置出现长短不一的循经高温反应(0.5～1.4℃)带或区,右侧多于左侧,而对照组则未显温度升高变化。模型组患侧和对侧循经高温反应部位的瘦素含量明显高于对照组(P<0.01)。另外,通过观察组织切片,在红外热像仪所测的高温反应皮下组织内,脂肪细胞多沿血管排列;对照组脂肪细胞的分布显得较疏松,而在模型组发生循经高温反应的组织内,脂肪细胞排列显得拥挤。结论:脂肪细胞分泌的瘦素与循经高温反应有一定相关性。

Objective To observe the content of leptin in the abdominal subcutaneous tissues of the higher temperature zone along the Gallbladder （GB） Meridian in cholecystitis rabbits so as to explore the correlation between the temperature response and the leptin level. Methods Twenty-five rabbits were randomized into normal control group （n = 10） and cholecystitis model group（n = 15）. Cholecystitis model was established by injection of Escherichia coli solution （0. 1 mL） into the gallbladder via common bile duct after surgery. Three, 7 and 11 days following modeling, the abdominal surface thermographic images along the GB Meridian were collected by the AGA-782-type infrared imaging system （AGA＇s capacity） . And then the subcutaneous tis- sues of the high temperature reaction region and the contralateral corresponding tissues of different groups were collected for assa- ying the leptin contents in the supernatant by radioimmunoassay after processing （homogenization and centrifugation） and for ob- serving the morphological structure under light microscopy after sectioning and staining （toluidine blue）. Results Thermographic images showed that about 70% of the animals in the model group, various lengths of high temperature reaction （0.5-- 1.4 （3 ） zones or bands in the abdominal region along the bilateral GB Meridian were seen, in comparison with the control group. The leptin levels in the ipsilateral （affected side） and contralateral （healthy side） tissues of the abdominal high temperature reaction zones along the GB Meridian were significantly higher in the model group than in the control group （P〈0.01）, suggesting a close correlation between the leptin content change and the high temperature reaction. In addition, most fat cells were crowded to dis- tribute closely along the blood vessels in the subcutaneous tissues of the higher temperature reaction zone in the model group, but those of the control group distribute more loosely along the blood vessels. Conclusion The leptin content in the abdominal subcutaneous tissues of high temperature reaction zone along the GB Meridian is higher in cholecystitis rabbits, suggests a relevance of the endocrine function of fat cells to the high temperature reaction along the GB Meridian.