针刺俞、募穴对功能性消化不良大鼠血清大分子代谢产物的影响

Effects of Electroacupuncture of "Weishu" (BL 21)and "Zhongwan" (CV 12) on Serum Large Molecular Metabolites in Functional Dyspepsia Rats

目的:探讨针刺俞、募穴和配穴对功能性消化不良(FD)大鼠影响的部分代谢组学机制。方法:Wistar雄性大鼠50只,随机分为对照组、模型组、胃俞组、中脘组、胃俞联合中脘组,每组10只。采用夹尾法复制FD大鼠模型,分别电针"胃俞""中脘"以及"胃俞"+"中脘",采用1H NMR扩散编辑实验检测血清大分子代谢物,并进行主成分分析和正交偏最小二乘判别分析。结果:与对照组相比,模型组大鼠血清中N-乙酰糖蛋白(NAc)和高密度脂蛋白(HDL)含量明显降低,极低密度脂蛋白/低密度脂蛋白(VLDL/LDL)明显升高(P<0.05);针刺各组均能提高NAc的含量,降低VLDL/LDL(P<0.05);与胃俞组和中脘组相比,胃俞+中脘组除了升高NAc,降低VLDL/LDL之外,还能提高模型大鼠降低的HDL(P<0.05)。结论:针刺俞募穴均对FD大鼠的血清大分子代谢产物有影响,俞募配穴法的影响更为明显。

Objective To compare the effects of electroacupuncture （EA） of Shu-acupoint ＂Weishu＂ （BL 21）, Mu-acupoint ＂Zhongwan＂ （OV 12） and BL 21 ＋ CV 12 on serum large molecular metabolites in functional dyspepsia （FD） rats. Methods A total of 50 Wistar rats were randomly divided into control, model, BL 21, OV 12 and BL 21 ＋ CV 12 groups （n = 10/group）. FD model was established by clamping the rats＇ tails for 30 min, once every 3 h, 4 times daily for 7 days. EA was applied to BL 21,0V 12, or BL 21 ＋OV 12, respectively for 20 min, once daily for 10 days. The rats＇ venous blood was collected for detecting large molecular metabolites by ~H nuclear magnetic resonance （NMR） spectrometer （Varian Unitylnova, 600 MHz）. Segmental integral calculus （0.04 ppm per segment） was performed from 6.0-0 ppm （longitudinal eddy-delay, LED） as defaulted H spectra values by using the VNMR software. Data was saved as text or excel files after normalization and then used for pattern recognition analyses. All the data were analyzed by principal component analysis （POA） using the SlMOA-P 10. 04 software （Umetrics AB, Umea, Sweden）, and orthogonal signal correction and partial least squares discriminant analysis （OSO-PLS-DA）. Results The rat serum proton-LEDJ H NMR spectra revealed different metabolic spectra among control,model, BL 21 ,CV 12, and BL 21＋ OV 12 groups. POA and OSO-PLS-DA showed that compared with the normal control group, FD rats in model group displayed serum N-acetylglucoprotein （NAc） content was reduced considerably, high density lipoprotein （HDL） level decreased to a certain degree, while very low density lipoprotein （VLDL） / LDL increased significantly. In comparison with model group,VLDL/LDL was decreased in BL 21, CV 12 and BL 21 ＋CV 12 groups, while NAc content increased. Compared with BL 21 and CV 12 groups respectively, HDL level in BL 21 ＋ CV 12 increased to a certain degree. Conclusion EA of BL 21, CV 12 and BL 21 ＋ CV 12 groups can reverse or relieve FD induced increase of VLDL/LDL and decrease of serum NAc level in rats, which may contribute to its function in improving symptoms of FD patients.