84KD精子膜蛋白与抗精子抗体

A 84 KD SPERM MEMBRANE PROTEIN INDUCES HIGH TITER ANTISPERM ANTIBODIES

为了进一步研究精子抗原及其相应抗体在不育中的作用,本文用SDS-PAGE和免疫印迹法检查了一对长期不育夫妇之间的抗精子免疫应答。该夫妇双方均含高滴度抗精子抗体。丈夫精子膜蛋白提取液中分子量84KD蛋白含量显著增加,为正常男性的3～5倍。该成份在免疫印迹中可被夫妇双方血清特异性识别。提示不育男性精子抗原成份与生育力正常男子有量或质的差异,可能是产生抗精子抗体并造成不育的一个原因。

An unusually high immunological response to sperm in an infertile couple was inves-tigated using the husband's sperm and their sera by SDS-PAGE and Western blotting. Thewife had been infertile for more than 8 years with a high titer of sperm agglutinating an-tibody (up to 1: 16000). The husband's semen showed positive reaction for antisperm an-tibodies by direct immunobead assay. The content of a 84 KD molecular weight glycoproteinin the husband's sperm extract was up to 3-5 times of immunoblotting test.that found in fertile men. This protein was by both the husband's and wife's sera inThese findings provided evidence that sperm antigen components of some infertilemen may be different from those of fertile men. Antisperm antibodies induced by theseantigens in the man and his partner may be one of the causal factors of their infertilestatus.