细胞质胸苷激酶在维吾尔族妇女宫颈癌的表达及临床意义

Expression of TK1 in cervical carcinoma in Uighur women and its clinical significance.

目的:探讨细胞质胸苷激酶(TK1)在宫颈鳞癌的表达及其临床意义。方法:制备40例维吾尔族妇女子宫颈癌及正常宫颈组织中总RNA,用RT-PCR方法分析TK1基因mRNA的表达水平,用免疫组化(SP)法检测其相应蛋白表达。结果:(1)维吾尔族妇女宫颈癌组织中TK1 mRNA及蛋白阳性检出率分别为67.5%及60.5%,对照组宫颈组织中检出率为26.7%及8.3%。宫颈癌组织中TK1 mRNA及蛋白阳性表达明显高于对照组的宫颈组织(P<0.05);(2)在宫颈癌组织中TK1 mRNA及蛋白表达:不同临床分期及分化程度之间无显著差异(P>0.05),但是,有淋巴结转移患者组织中阳性表达明显高于无淋巴结转移者,肿瘤直径≥4cm患者阳性表达明显高于肿瘤直径<4cm的患者(P<0.05)。结论:TK1在维吾尔族妇女宫颈鳞癌中高表达,对宫颈癌的诊断有重要的参考价值,TK1高表达可能与宫颈癌的进展及预后有关。

Objective：To explore the expression of TK1 mRNA and protein and its significance in cervical carcinoma in Uighur women.Methods：TK1 mRNA expression were evaluated by RT-PCR and TK1 protein expression were detected by immunohistochemistry method（SP） in 40 cases of cervical carcinoma in Uighur women.Results：（1）The positive expression rates of TK1mRNA and TK1 protein in cervical carcinoma were 67.5% and 60.5%,which were significantly higher than 26.7% and 8.3% in control group.（2）The positive expression rates of TK1mRNA and TK1 protein were obviously higher in lymph node metastatic group and the group with tumor diameter more than 4cm than non-metastatic group and the group of less than 4cm,respectively（P0.05）,while no significant differences between different clinical stages and pathological grades（P0.05）.Conclusion：TK1 has a substantial diagnostic value for cervical carcinoma in uighur women,which may be related to its progress and prognosis.