摘要
目的:探讨细胞质胸苷激酶(TK1)在宫颈鳞癌的表达及其临床意义。方法:制备40例维吾尔族妇女子宫颈癌及正常宫颈组织中总RNA,用RT-PCR方法分析TK1基因mRNA的表达水平,用免疫组化(SP)法检测其相应蛋白表达。结果:(1)维吾尔族妇女宫颈癌组织中TK1 mRNA及蛋白阳性检出率分别为67.5%及60.5%,对照组宫颈组织中检出率为26.7%及8.3%。宫颈癌组织中TK1 mRNA及蛋白阳性表达明显高于对照组的宫颈组织(P<0.05);(2)在宫颈癌组织中TK1 mRNA及蛋白表达:不同临床分期及分化程度之间无显著差异(P>0.05),但是,有淋巴结转移患者组织中阳性表达明显高于无淋巴结转移者,肿瘤直径≥4cm患者阳性表达明显高于肿瘤直径<4cm的患者(P<0.05)。结论:TK1在维吾尔族妇女宫颈鳞癌中高表达,对宫颈癌的诊断有重要的参考价值,TK1高表达可能与宫颈癌的进展及预后有关。
Objective:To explore the expression of TK1 mRNA and protein and its significance in cervical carcinoma in Uighur women.Methods:TK1 mRNA expression were evaluated by RT-PCR and TK1 protein expression were detected by immunohistochemistry method(SP) in 40 cases of cervical carcinoma in Uighur women.Results:(1)The positive expression rates of TK1mRNA and TK1 protein in cervical carcinoma were 67.5% and 60.5%,which were significantly higher than 26.7% and 8.3% in control group.(2)The positive expression rates of TK1mRNA and TK1 protein were obviously higher in lymph node metastatic group and the group with tumor diameter more than 4cm than non-metastatic group and the group of less than 4cm,respectively(P0.05),while no significant differences between different clinical stages and pathological grades(P0.05).Conclusion:TK1 has a substantial diagnostic value for cervical carcinoma in uighur women,which may be related to its progress and prognosis.
出处
《现代妇产科进展》
CSCD
北大核心
2010年第8期601-604,共4页
Progress in Obstetrics and Gynecology
基金
新疆维吾尔自治区重点实验室基金项目(No:XJDX0208-2006-03)