肝素涂层体外循环管道中聚乙烯亚胺的作用研究

Effects of concentration and molecular weight of polyethyleneimine on heparin-coated modified polyvinyl chloride material

目的:探讨聚乙烯亚胺(PEI)的浓度、平均相对分子量对涂层肝素的影响,筛选出合适的各种底物的浓度及反应条件,优化涂层工艺。方法:将PEI的平均相对分子量(3 000、10 000、20 000)和质量浓度(1 g/dl、2 g/dl、5 g/dl)交叉组合分为9个组,制成PEI-戊二醛-肝素涂层体外循环管道。利用红外光谱对结合肝素的表征定性,用甲苯胺蓝分光光度法测量肝素的结合含量。结果:通过PEI-戊二醛-肝素涂层体外循环管道肝素的结合量,受PEI的分子量及浓度的影响,最适质量浓度及平均相对分子量分别为2 g/dl和20 000。结论:各组肝素涂层材料肝素的浓度随PEI平均相对分子量的递增而增加,在一定范围内,PEI的浓度与肝素的含量呈正相关,浓度过高或过低均不利于肝素的结合。

AIM： To study the effects of concentration and molecular weight of polyethyleneimine （PEI） on PEI-heparin-coated polyvinyl chloride （PVC） medical materials. METHODS： Using PEI-glutaralde- hyde-heparin coating techniques, cardiopulmonary bypass （CPB） circuits were coated with heparin and then divided into nine groups by PEI concentrations and molecular weights. The content of the coated heparin was calculated by toluidine blue spectrophotometry. RESULTS： The combination of the amount of heparin on PEI-heparin-coated PVC medical material was affected by the concentration and molecular weight of PEI. The optimal concentration and molecular weight of PEI were 2 g/dl and 20 000, respectively. CONCLUSION： Heparin concentration on PEI-glutaraldehyde-heparin-coated PVC surface is increased by the concentration and molecular weight of PEI. The increase is to some degree positively related to the content of PEI. Too high or too low PEI concentrations are not conducive to the combination of heparin.