摘要
本文用精子活动轨迹追踪摄片法对9例健康有生育力的正常人精液样本分别加入不同浓度Ca^(2+)和EGTA^(2-)观察它们对精子活力的影响。结果表明增加Ca^(2+)或Ca^(2+)被EGTA^(2-)螯合而减少均可影响精子的活力,并且精子失活率随Ca^(2+)或EGTA^(2-)的浓度增加而增加,加入Ca^(2+),精子失活率增加的速度比加入EGTA^(2-)快。加浓度为127.32mMCaCl_2于人精液一分钟,精子失活率可达100%。因此钙可能在发展新的避孕药方面有一定的价值,但这样浓度的钙盐对人机体细胞有无不良影响还需作进一步研究。
This paper introduces the investigation of the effect of various concentration of Ca^(2+)& EGTA^(2-) on the spermatozoal motility of 9 healthy fertile donors with the aid ofmultiple exposure photography. The results showed that the increasing of spermatozoal im-motility rate was in keep with the progressively increasing the concentration of Ca^(2+) orEGTA^(2-). But increasing the concentration of Ca^(2+) would speed up the immobllity rate ratherthan increasing the EGTA^(2-) and the calculated results also showed that the immobilityrate might be 100% by adding 127.32 mM CaCl_2 to the specimen of human semen for1 minute. It reveals that ealcium may be effcotive in the development of new contraceptivesyet it has to be studied whether there is any harm to human tissue at such a concentra-tion of CaCl_2.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1990年第3期30-34,共5页
Reproduction and Contraception
关键词
人
钙离子
EGTA离子
螯合剂
精子
Human sperm
Ionized calcuim
Ionized EGTA Spermatozoal immotility rate
Mutiple exposure photography