糖脂平颗粒对糖尿病胰岛素抵抗大鼠血脂及游离脂肪酸的影响

Effect of Tangzhiping Granule on Blood Lipids and Free Fatty Acids in Rats with Insulin Resistant Diabetes

目的探讨糖脂平颗粒对糖尿病胰岛素抵抗大鼠血脂及游离脂肪酸的影响。方法随机抽取正常大鼠为正常对照组,剩余大鼠使用高脂高糖饲料和注射小剂量链脲佐菌素诱导糖尿病胰岛素抵抗大鼠模型。选取造模成功大鼠32只,随机分为模型组、糖脉康颗粒组、糖脂平大、小剂量组,每组8只,分别予以等体积生理盐水、糖脉康颗粒(1.35 g/kg)、糖脂平颗粒大(2.70 g/kg)、小剂量(1.35 g/kg)灌胃治疗4周。检测各组大鼠体质量、血糖(FBG)、血清胰岛素(INS)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及游离脂肪酸(FFA),并计算胰岛素敏感性指数(ISI)。结果各治疗组治疗后体质量均低于模型组,差异有统计学意义(P<0.05);各治疗组治疗后FBG均低于模型组,差异有统计学意义(P<0.05),其中糖脂平大剂量低于小剂量组和糖脉康组,差异有统计学意义(P<0.01);各治疗组与模型组比较INS均明显下降,血清ISI值均明显升高(P<0.01),糖脂平大剂量组与糖脂平小剂量组、糖脉康组比较,INS升高、ISI值降低更为明显(P<0.01);各治疗组与模型组比较,TG、TC、LDL-C、FFA降低,HDL-C升高,差异均有统计学意义(P<0.05),糖脂平大剂量组疗效优于小剂量组和糖脉康组(P<0.05)。结论糖脂平颗粒可降低2型糖尿病胰岛素抵抗大鼠体质量、FBG、INS、TC、TG、LDL和FFA,同时可升高ISI值和HDL-C,从而改善了2型糖尿病模型大鼠的胰岛素抵抗。

Objective To investigate the effects of Tangzhiping Granule（TZPG） on blood lipids and free fatty acids（FFA） in rats with insulin resistant diabetes（IRD）.Methods A blank control group consisted of randomly selected normal rats was set up.The remaining rats were established to IRD model by high-fat high-sugar diet feeding and streptozotocin injection.Then the 32 successfully modeled rats were randomized into the model group（treated by saline）,the Tangmaikang group（treated with Tangmaikang Granule 1.35 g/kg）,and the two TZPG groups treated with high dose（2.70 g/kg） and low dose TZPG（1.35 g/kg） respectively through intragastric infusion for 4 weeks.The body weight（BW）,fasting blood glucose（FBG）,insulin（INS）,blood lipids including triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,and FFA were detected,and the insulin sensitivity index（ISI） calculated.Results Compared with the blank control group,BW,FBG and INS increased while ISI decreased in the model group（P0.05 or P0.01 ）.All the above-mentioned abnormal indices were improved in the three treated groups （Tangmaikang,high and low dose TZPG group）,but the improvements in the high dose TZPG group were more significant than those in the other two groups（P0.05 or P0.01）.Similar outcomes were also seen in blood lipids detection,in which TG,TC,LDL-C and FFA were higher and HDL-C were lower in model rats than those in blank controls,they were improved in the three treated groups（P0.05）,and the best improvements were seen in the high dose TZPG group（P0.05）.Conclusion TZPG could reduce levels of BW,FBG,INS,TC, TG,LDL-C and FFA,and increase levels of ISI and HDL-C in rat model of insulin resistant type 2 diabetes,so as to improve the insulin resistance in them.