不同局部穴位电针对大鼠受损伤脊髓组织降钙素基因相关肽表达的影响

Effects of different local points of electroacupuncture on the expression of calcitonin gene-related peptide in rats with spinal cord injury

目的:观察不同局部穴位电针对大鼠受损伤脊髓组织降钙素基因相关肽CGRP表达和分布的影响。方法:将25只SD大鼠分为5组:正常对照组、脊髓损伤组、非穴位电针组、夹脊穴电针组和督脉穴电针组。在显微镜下予脊髓全横断术(正常对照组除外),自术后第7天分别采用局部选取电针非穴位、电针夹脊穴和电针督脉穴的方法治疗3d(脊髓损伤组不予任何治疗),取材并以免疫荧光组织化学染色的方法观察脊髓背角CGRP阳性染色区面积变化,用蛋白免疫印迹杂交法检测脊髓CGRP含量变化。结果:非穴位电针组和夹脊穴电针组的脊髓背角CGRP含量与脊髓损伤组比较,均有所升高(P<0.05),但其CGRP阳性染色区面积没有明显差异(P>0.05)。督脉穴电针组的脊髓背角CGRP含量和CGRP阳性染色区面积与非穴位电针组、夹脊穴电针组和脊髓损伤组比较,有明显增高、增大(P<0.05)。结论:不同局部穴位电针对大鼠受损伤脊髓组织表达CGRP有不同的影响,其中督脉穴电针能够明显促进大鼠受损伤脊髓组织表达CGRP。

Objective：To observe the effects of electroacupuncture（EA） at different local points on the expression and distribution of calcitonin gene-related peptide（CGRP） in the rats with spinal cord injury（SCI）.Method：Twenty-five SD rats were divided into five groups：normal control group,SCI group,non-point area（npa）EA group,Jiaji-point（Jiaji）-EA group and DU-meridian point（DUmp）-EA group.The SCI model was estublished by spinal cord transection under microscope.The treatment of npa-EA,Jiaji-EA and DUmp-EA were applied for 3 days from the 7th day after operation（SCI group no treatment）.The spinal cord tissues were taken out and stained with immunofluorescence histochemistry technique to observe the changes of CGRP-positive area in spinal cord dorsal horn.The changes of contents of CGRP in spinal cord were detected by Western blot technique.Result：Compared with SCI group,the contents of CGRP in npa-EA and Jiaji-EA groups were higher（P0.05）,but there was no statistically significant difference in CGRP-positive staining areas in spinal cord dorsal horn（P0.05）.In DUmp-EA group CGRP-positive staining areas in spinal cord dorsal horn were larger and contents of CGRP in spinal cord were higher than those in npa-EA group,Jiaji-EA group and SCI group（P0.05）.Conclusion：EA at different local points had different effect on the expression of CGRP in rats with SCI andDUmp-EA can obviously promote the expression of CGRP in rats with SCI.