孝顺竹愈伤组织增殖培养基优化研究

Medium Optimization for Callus Proliferation of Bambusa multiplex

为筛选适宜孝顺竹愈伤组织继代增殖培养基,控制褐变发生,提高再生体系效率,对培养基组成如5种基本培养基、6种有机添加物、7种糖类和5种大量元素等因子进行试验分析。结果表明:培养20 d,基本培养基以MS效果较好,愈伤组织增殖2.8倍,白至淡黄色,致密;有机添加物以1.0 g.L-1脯氨酸效果较显著,愈伤组织增殖3.64倍,淡黄色,致密均一;碳源以30 g.L-1麦芽糖效果较好,愈伤组织增殖2.96倍,白至淡黄色,致密。5种大量元素中NH4NO3对孝顺竹愈伤组织增殖的影响达到显著水平,以825 mg.L-1为较佳浓度,培养29 d愈伤组织增殖可达5倍以上,部分出现根分化;适宜孝顺竹愈伤组织培养的大量元素组合为:KNO3475 mg.L-1+NH4NO3825mg.L-1+MgSO4.7H2O 185 mg.L-1+KH2PO4340 mg.L-1+CaC l2.7H2O 440 mg.L-1。

An effective regeneration system is the pre-requisite for genetic transformation of bamboos.Medium components for callus proliferation of Bambusa multiplex were studied,5 basal medium,6 organic additives,7 sugars,and 5 macronutrients were compared.The results showed that Murashige and Skoog（MS） basal medium was effective,callus increased 2.8 times at 20 days,white to yellowish,compact;1.0 g·L-1 proline was suitable the organic additive,callus increased 3.64 times,yellowish,compact and even;30 g·L-1 maltose was the optimal carbon source,callus increased 2.96 times,white to yellowish,even.Among five macronutrients,NH4NO3 has significant influence on callus proliferation（α=0.05） and its optimal concentration was 825 mg·L-1,callus could increase over 5 times at 29 days,with some rooting;KNO3 475 mg·L-1＋NH4NO3 825 mg·L-1＋MgSO4·7H2O 185 mg·L-1＋KH2PO4 340 mg·L-1＋CaCl2·7H2O 440 mg·L-1 is a suitable combination for callus proliferation.