摘要
微卫星标记(SSR)是分子育种中常用的遗传标记之一,但目前报道的番茄SSR标记并不能满足番茄分子育种的需要,有必要发展出更多的SSR标记,近缘物种转移法是一种发展SSR标记简便快速的方法。本研究分析了茄子SSR标记在番茄上的通用性情况,结果表明:300对茄子SSR引物中有111对能在番茄基因组DNA上扩增出产物,97对引物扩增出的带型在茄子、番茄间相似程度高,标记通用率为32.3%;EST-SSR比基因组SSR的通用性更好,前者通用率为36.6%,后者为30.0%。将这些标记转移到番茄上,能节省开发番茄SSR标记的成本。
Simple sequence repeats（SSR） is one of the marker systems widely used in molecular breeding.However,only a limited numberof SSRs have been reported in tomato by now and cannot satisfy the demand of molecularbreeding,it is still necessary to develop more SSRs in tomato.SSR transfer between related species is a feasible method to develop new SSR markers.The current study has tested the transferability of eggplant SSRs to tomato.A total of 300 eggplant SSRs were tested,111 of which could successfully amplified on tomato,but only 97 SSRs could produce similarbands on tomato and eggplants,giving a transfer percentage of 32.3%.EST-SSRs had higher transferability than genomic SSRs,which were 36.6% and 30.0%,respectively.This study added up the numberof robust SSRs in tomato by an economic method.
出处
《分子植物育种》
CAS
CSCD
2010年第5期909-912,共4页
Molecular Plant Breeding
基金
国家自然科学基金(30771472
30911120052)
广东省科技厅国际合作项目(2009B050100001)
科技部国际合作项目(2010DFA32190)共同资助
关键词
茄子
番茄
微卫星标记
通用性
Eggplant
Tomato
Simple sequence repeats（SSR）
Transferability
