核桃早实性相关的SCAR标记在母本和F_1代上的分析

Analysis of the molecular maker related to Early-seeding in walnut female parent and F_1 by SCAR

本文研究目的是对与新疆核桃早实性相关的SCAR标记在母本和F1代上进行分析。通过对与核桃早实性相关的SCAR标记片段的扩增并测序,获得与早实性相关的SCAR标记片段,大小为762bp。用该特异性引物对新疆五个核桃实生品种母本和F1代进行扩增,在母本和F1代上得到了以下的结果:四个母本材料出现了该标记片段,各品种实生F1代单株出现该标记的比率分别为92.68%,97.92%,96.43%,93.10%和97.73%,分析表明核桃早实性是遗传力较高的性状。本研究可为进一步研究新疆核桃早实性相关基因的克隆及功能提供一定的研究基础。

In this study,we analyzed the heredity rule of SCAR molecular marker related to early-bearing trait in maternal parents and F1 generations of walnut in Xinjiang.By PCR amplification and sequencing,the SCAR molecularmarkerrelatedto early-bearing trait in walnut was obtained,which was 762 bp.PCR amplification of the maternal parents andtheirF1 generations showedthatthe fourmaternal parents testedall obtaineda 762 bp band.The percentage of F1 generations containing this markerwas 92.68%,97.92%,96.43%,93.10%and 97.73%,respectively.The result indicates that the early-bearing trait was a trait with high heredity ability.This study would provide us some insights forstudying early-bearing walnut of Xinjiang province in the future.