人胚胎脑提取液的运动神经营养活性研究

Study on motoneuron neurotrophic activity of the extract of embryo brain

目的 从人胚胎脑中提取对运动神经元具有生物活性的神经营养因子。方法 用人胚胎脑组织制备提取液,以成年Wistar大鼠脑提取液及生理盐水为对照,在切断坐骨神经的乳鼠动物模型上,检测运动神经营养活性。结果 在损伤侧局部注射提取液,术后30天损伤侧腰髓运动神经元存活率;第2月人胚脑提取液组为81.4％;第4～5月胎儿脑提取液组为80.7％;成年大鼠脑提取液为50.7％;生理盐水空白对照组为25％。结论 人胚胎脑提取液与两对照组相比具有明显的运动神经营养活性。

Objective To extract the neurotrophic factors with motoneuron neurotrophic activity from the brains of embryo. Methods The extract was prepared from the brains of embryo. The extract of adult rat and physiological saline were treated as control groups. The motoneuron neurotrophic activities of those extract were detected in sciatic nerve injured models of neonatal Wistar rats of 3 day age. Results Thirty days following resection of sciatic nerve, by multiple injection of the second months embryo's brain extracts at the site of axotomy, 81.4% of the corresponding motoneurons were protected from cell death. The motoneurons survival rate was 80.7% when treated with 4-5 months foetus' brain extracts and 50. 7% of adult rats' brain extracts. In blank control (injecting physiological saline), there are only 25% of the corresponding motoneuron can survive. Conclusion The result indicated that the brain extract of the second months embryo and 4-5 months foetus all showed remarkable motoneuron neurotrophic activities compared with two controls.