重型乙型肝炎患者血清白细胞介素-8的检测及其临床意义

Clinical significance of change in serum interleukin8 levels in patients with severe viral hepatits B

目的：探讨血清白细胞介素８（ＩＬ８）在重型乙型肝炎患者中的变化及其临床意义。方法：采用双单克隆抗体夹心酶联免疫吸附（ＥＬＩＳＡ）法检测３２例重型乙型肝炎患者血清ＩＬ８水平，并以４０例正常人和２０例乙型慢性活动性肝炎患者作对照。结果：重型乙型肝炎患者血清ＩＬ８水平〔（１．１２６±０．４３１）ｍｇ／Ｌ〕显著高于正常人〔（０．０５０±０．００４）ｍｇ／Ｌ，Ｐ＜０．００１〕和乙型慢性活动性肝炎患者〔（０．６３７±０．２１３）ｍｇ／Ｌ，Ｐ＜０．０１〕，其升高程度与血清胆红素含量及凝血酶原活动度变化密切相关。血清胆红素含量愈高和凝血酶原活动度愈低，血清ＩＬ８水平愈高。重型乙型肝炎合并细菌感染患者ＩＬ８〔（１．５１３±０．２６２）ｍｇ／Ｌ〕高于无细菌感染者〔（０．９１２±０．２１４）ｍｇ／Ｌ，Ｐ＜０．０５〕，重叠肝炎病毒感染时血清ＩＬ８水平〔（１．６１２±０．３８１）ｍｇ／Ｌ〕比单纯重型乙型肝炎患者〔（０．９３１±０．３１２）ｍｇ／Ｌ〕显著升高（Ｐ＜０．０５）。死亡病例血清ＩＬ８水平〔（２．１２４±０．７３１）ｍｇ／Ｌ〕比生存者〔（０．９９７±０．６７５）ｍｇ／Ｌ〕显著升高（Ｐ＜０．０１）。结论：检测血清ＩＬ８水平对评估?

Objective:To investigate the change in serum interleukin8(IL8) levels and its clinical significance in severe viral hepatitis B.Methods:Serum IL8 levels were measured with sandwich enzyme linked immunosorbent assay (ELISA) in 32 patients with severe viral hepatitis B(SH),and 40 normal controls (NC) and 20 patients with chronic active hepatitis B(CAH).Results:Serum IL8 levels in severe viral hepatitis B were obviously increased (1 126±0 431)mg/L compared to NC (0 050±0 004)mg/L, P <0 001 or CAH (0 637±0 213)mg/L, P <0 01 ,and it was correlated with serum bilirubin concentrations or prothrombin activity.The higher the serum bilirubin and the lower the prothrombin activity were,the higher would be the serum IL8 levels.Also,serum IL8 levels in SH with bacterial infections were marked elevated compared to those without bacterial infections (1 513±0 262)mg/L vs.(0 912±0 214)mg/L, P <0 05 .Similarly,serum IL8 levels in nonsurvivors were significantly higher than survivors (2 124±0 731)mg/L vs.(0 997±0 675)mg/L, P <0 01 .Conclusions:Those findings suggest that measurement of serum IL8 is helpful in evaluating the clinical severity and in assessing the prognosis of the disease.