6周递增负荷运动后大鼠脾淋巴细胞分泌IL-2和sIL-2R表达的适应性特征

6 Weeks Incremental Exercise Later the Adaptive Characteristics of IL-2 and SIL-2R of Lymphocyte from Rat Spleen

用72只雌性SD大鼠进行六周递增负荷运动训练,分别在第0、2、4、6周末即(WK0、WK2、WK4、WK6)定量负荷运动前(A)、运动后即刻(J)、运动后3h(3h)取材,用ELISA法测试脾淋巴细胞培养上清中白细胞介素2(IL-2)和可溶性白细胞介素2受体(sIL-2R)浓度,试图在细胞水平观察IL-2和sIL-2R在整个免疫过程中的分泌功能和受体表达变化,以此来分析运动影响免疫功能的机理。结果表明:通过递增负荷运动训练后大鼠脾淋巴细胞分泌IL-2和sIL-2R的表达都呈现降低-升高-再降低的应答性变化,但IL-2分泌量在WK0水平上下波动,而sIl-2R表达量则在WKO水平下波动。结论:IL-2和sIL-2R变化的不同步导致了免疫功能低下。免疫功能的降低主要是由于sIL-2R表达的降调节导致,但机体以IL-2的大量分泌来尽量维持机体的免疫功能。

We used 72 female rats jogging in the treadmill for six weeks under incremental exercise increase progressively by 5m/min each week.The spleen was taken before,just after and three hours after exercise respectively in 0W,2W,4W,6W weekend.We used the ELISA to detect the concentration of IL-2 and sIL-2R trying to observe the responsibility and recovery of IL-2 and sIL-2R in incremental exercise.The result was that after six weeks increased progressively training,IL-2 and sIL-2R had some definite responsibility relationship.Inclusion： IL-2 and sIL-2R changes were not synchronized led to immune function.Reduced immune function mainly dued to the expression of sIL-2R down-regulation lead,but the body to secrete IL-2 to try to keep a large number of immune function.