养阴益气活血法改善2型糖尿病胰岛素抵抗的机制研究

Mechanism on yiqiyangyinhuoxue therapy’s improvement on type 2 diabetes’ insulin resistance

目的观察具有养阴益气活血作用的中药复方制剂参丹健胰丸对2型糖尿病模型大鼠胰岛素抵抗的改善作用,并探讨其机理。方法采用高热量饲料加小剂量链脲佐菌素建立类似于2型糖尿病胰岛素抵抗的大鼠模型,通过设立正常对照组、模型对照组、参丹健胰丸1 080 mg·kg^-1治疗组、参丹健胰丸540 g·kg^-1治疗组、二甲双胍治疗组和参芪降糖颗粒组,观察各组大鼠治疗前后空腹血糖（FBG）、血清胰岛素水平（Fins）、胰岛素敏感指数（ISI）、血清C反应蛋白（CRP）、血浆纤溶酶原激活物抑制物（PAI-1）、血清游离脂肪酸（FFA）以及血清肿瘤坏死因子（TNF-α）水平。结果参丹健胰丸组大鼠FBG、血浆PAI-1活性及血清Fins、CRP、FFA、TNF-α含量均较模型组大鼠明显降低（P〈0.05或P〈0.01）,ISI明显增加（P〈0.05或P〈0.01）。结论养阴益气活血中药复方参丹健胰丸对2型糖尿病大鼠胰岛素抵抗具有一定的改善作用。

Abtract： Aim To investigate the effect and probe into the mechanism of shendanjianyi capsule on insulin resistance in type 2 diabetic rats.Methods The model of type 2 diabetes mellitus rats were established with feeding high sucrose-fat diet and injecting small dose of streptozotocin（STZ）.All rats were divided randomly into 6 groups： normal control group,model control group,shendanjianyi capsule 1 080 mg·kg^-1 group and 540 mg·kg^-1 group,diformin tablet group,shenqijiangtang pellet group.The level of serum insulin,plas-minogen activator inhibitor-1,C-reactive protein,free fatty acid,tumour necrosis factor-α etc.were examined after treatment.Results The levels of fasting blood glucose,plasminogen activator inhibitor-1,serum insulin,serum C-reactive protein,serum free fatty acid and serum tumour necrosis factor-α of rats in shendanjianyi capsule group were all lower those that in model control group（P 〈0.05 or P 〈0.01）,and the level of serum insulin sensitivity index of rats in shendanjianyi capsule group was higher than that in model control group（P 〈0.05 or P〈 0.01）.Conclusion Shendanjianyi capsule can ameliorate the insulin resistance of model rats.