毛发上皮瘤与角化型基底细胞癌细胞形态及DNA含量定量分析

Morphology of the Cell and Quantitative Analysis of DNA Content between Trichoepithelioma and Keratinizing Basal Cell.

目的利用体视学技术对毛发上皮瘤及角化型基底细胞癌组织病理相似部分基底样细胞瘤块进行细胞形态及细胞核ＤＮＡ含量分析。方法每组各选５例进行细胞及细胞核形态及大小的测定，各选３０例进行细胞核ＤＮＡ含量定量分析。结果细胞形态及大小两组无差异（Ｐ＞０．０５），细胞核形态及大小的各参量值两组有显著性差异（Ｐ＜０．０５）。毛发上皮瘤组３０例均为二倍体，角化型基底细胞癌组有异倍体１６例。结论两组肿瘤细胞的形态及大小相似，但角化型基底细胞癌的细胞核相对较大，且不规整，显示了恶性肿瘤的形态学特征。角化型基底细胞癌呈恶性生物学行为，而毛发上皮瘤则呈良性。

Objective Sterelology method was applied to study the morphology of the cell and analyze quantitatively the nuclear DNA content of the basal cells from Trichoepithelioma and Keratinizinng Basal Cell Epithelioma.Methods Five specimens embedded by paraffin from each group were used to measure the cell shape and size,30 specimens were used to annalyze quantitatitvely DNA content of the nuclei.Results The difference between the two groups for cell shape and size did not reach statistical significance( P >0.05).Data for nucleus shape and size were significantly higher in keratinizing basal cell epithelionma than in trichoepithelioma( P <0.05).Quantitative analysis of DNA content showed 30 speciments from trichoepithelioma were all diploid.While 16 of 30 specimens from keratinizing basal cell epithelioma were aneuploid.Conclusion Although both tumor cells appear alike in cell shape and size,tumor nuclei from keratinizing basal cell epithelioma were larger and atypical.It showed the cytomorphology and the bilogical behavior of malignant tumor.Comparatively the trichoepithelioma is of benign biological behavior.