恶性小汗腺汗孔瘤的免疫组化和超微结构观察

Immnohistochemical and Ultrastructural Observation on Malignant Eccrine Poroma.

目的观察细胞角蛋白在恶性小汗腺汗孔瘤中的表达，以期探讨其组织发生。方法采用免疫组化方法对６例恶性小汗腺汗孔瘤的石蜡包埋标本进行检测，并对其中２例进行透射电镜观察。结果绝大多数肿瘤细胞示３４βＥ１２阳性，部分肿瘤细胞（衬于导管样结构和囊状腔隙内的细胞）示３５βＨ１１、ＣＡＭ５．２和ＰＫＫ１阳性。电镜下可见肿瘤细胞胞质内管样结构。结论证实了恶性小汗腺汗孔瘤来源于小汗腺导管。同时提示，部分肿瘤细胞显示向小汗腺分泌部分化特征。

Objective To analyze cytokeratin expressions in malignant eccrine poroma in hopes of investigating its histogenesis.Methods 6 paraffin embedded samples of malignant eccrine poroma were studied immunohistochemically,and 2 cases were observed by TEM.Results Most tumor cells showed positive reaction for 34βE 12 .Some tumor cells (lining the ductal structures and cystic spaces)revealed positive staining for 35βH 11 ,CAM5.2 and PKK 1.Intracytoplasmic cavities were observed ultrastructurally.Conclusion Our findings inferred that malignant eccrine poroma derived from errine duct,and some tumor cells differentiated towards ecrrine secretory portion.