心理应激对实验性2型糖尿病倾向大鼠下丘脑室旁核c-fosmRNA和CRFmRNA表达的实验研究

Effect of psychological stress on expression of c-fosm RNA and CRF mRNA of paraventriculares nucleus of hypothalamus in rats with tendency of experimental diabetes 2

[目的]探讨心理应激诱发实验性2型糖尿病倾向大鼠发病的中枢机制及心身5号的调节作用。[方法]采用给大鼠腹腔注射小剂量链脲佐菌素(STZ),同时施予多项慢性心理应激(限制、旋转、拥挤)制作心理应激诱发实验性2型糖尿病大鼠模型。利用原位杂交技术,观测心理应激对糖尿病倾向大鼠下丘脑室旁核c-fosmRNA和CRFmRNA表达的影响。[结果]与正常组比较,单纯应激组c-fosmRNA和CRFmRNA表达增多(P<0.01),STZ+应激组大鼠c-fosmRNA和CRFmRNA表达增加更显著,与应激组和单纯STZ组相比均有差异(P<0.05或P<0.01)。与STZ+应激组比较,中药组两者表达均下降(P<0.05或P<0.01)。[结论]心理应激诱发实验性2型糖尿病大鼠发病的中枢机制与下丘脑室旁核c-fosmRNA和CRFmRNA表达密切相关,这可能是情志疾病基本病机气机失调的客观依据之一。

[Objective]To investigate the mechanism happened in CNS of rats having a tendency of type 2 diabetes induced by psychological stress and the regulatory effect of Shenxin No.5.[Method] The model was induced by intraperitoneal infusion of STZ in small dosage combined with multiple chronic psychological stimulations,including restriction,rotation and crowding.Using in situ hybridization the effect of psychological stress on the expression of c-fos mRNA and CFR mRNA of paraventriculares nucleus（PVN）of hypothalamus was observed.[Results]Compared with the normal group,the transcription level of both c-fos mRNA and CFR mRNA in stimulation only group was increased（P0.01）. The group of STZ plus stimulation was considerably higher than other group（P0.05 or P0.01）.In Chinese herbs group both expression was all decreased（P0.05 or P0.01）.[Conclusion]The CNS mechanism of the onset of type 2 diabetes induced by psychological stress was closely connected with the expression of c-fos mRNA and CRF mRNA.That maybe one of the objective evidences of the disorder of Qi movement of emotional disease.