分子标记辅助选择改良圣稻13和圣稻14的条纹叶枯病抗性

Improving Resistance of Japonica Varieties Shengdao13 and Shengdao14 to Rice Stripe Virus Disease by Molecular Marker-Assisted Selection

【目的】利用分子标记辅助选择改良圣稻13、圣稻14的条纹叶枯病抗性,培育抗条纹叶枯病的粳稻新品种及新种质。【方法】以感病品种圣稻13、圣稻14为受体,以抗病品种镇稻88及高代抗病品系圣稻519为抗条纹叶枯病基因供体,采用自交改良和回交改良2种方法,利用与Stv-bi紧密连锁的分子标记H11-8、H11-12和H21进行辅助选择,结合条纹叶枯病田间抗性鉴定及农艺性状分析,将Stv-bi(或其等位基因)转移到圣稻13、圣稻14品种中,培育抗条纹叶枯病品种。【结果】通过2个自交混选群体的分子标记辅助选择获得多份稳定抗条纹叶枯病材料;通过回交改良,获得携带Stv-bi的圣稻13回交稳定品系E01、E13、R107等6个。【结论】利用分子标记辅助选择可显著提高选择效率和准确性,建立了抗条纹叶枯病分子标记辅助育种体系,为今后开展抗性育种提供了理论依据和材料基础。

【Objective】 To develop new varieties and germplasms resistance to rice stripe disease （RSD）,the molecular marker-assisted selection （MAS） strategy was used to improve the resistance of susceptible varieties Shengdao 13 and Shengdao14. 【Method】 Molecular markers closely linked to Stv-bi were used for MAS. Shengdao 13 and Shengdao14 were used as receptor parents,resistant variety Zhendao 88 and the high generation of disease-resistant rice strain Shengdao519 were used as the donor parents,applied self-improvement and backcross breeding methods,using MAS,combined with field resistance to RSD identification and agronomic traits analysis,Stv-bi （or allele gene） was successfully transferred to Shengdao13 and Shengdao14. 【Result】 A number of strains carrying the disease-resistance gene had been obtained through two inbred compound breeding populations,and six backcross stable lines carrying Stv-bi gene E01,E13,R107,etc. were obtained. 【Conclusion】 MAS can significantly improve the selection efficiency and accuracy in resistance breeding,MAS breeding system for stripe disease was established. The results have provided theories and materials for the future resistance breeding.